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Science 18 October 1996:
Vol. 274. no. 5286, pp. 433 - 0
DOI: 10.1126/science.274.5286.433e
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The Solid Phase Oligo COUPLE-IT Kit is for convenient coupling of primary amine-labeled oligonucleotides and other primary amine-labeled nucleic acids to a magnetic solid support. The kit contains all the reagents and magnetic solid support needed to carry out two coupling reactions. The resulting product can be stored for future use and re-used. The coupled product can be used in solid phase nucleic acid-based applications including reverse-transcriptase polymerase chain reaction, subtractive hybridization, purification of specific messages or RNA species, isolation of specific DNA molecules, hybridization-based assays, and purification and identification of nucleic acid binding proteins. Coupling with the magnetic solid support enhances the efficiency and convenience of downstream applications by enabling rapid, single tube assays and in most cases eliminating the need for centrifugation and column chromatography. CPG.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)