Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 3 October 2008:
Vol. 322. no. 5898, pp. 101 - 104
DOI: 10.1126/science.1161847
Prev | Table of Contents | Next

Reports

A Physical Map of the 1-Gigabase Bread Wheat Chromosome 3B

Etienne Paux,1 Pierre Sourdille,1 Jérôme Salse,1 Cyrille Saintenac,1 Frédéric Choulet,1 Philippe Leroy,1 Abraham Korol,2 Monika Michalak,3 Shahryar Kianian,3 Wolfgang Spielmeyer,4 Evans Lagudah,4 Daryl Somers,5 Andrzej Kilian,6 Michael Alaux,7 Sonia Vautrin,8 Hélène Bergès,8 Kellye Eversole,9 Rudi Appels,10 Jan Safar,11 Hana Simkova,11 Jaroslav Dolezel,11 Michel Bernard,1 Catherine Feuillet1

As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17–billion–base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)–based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.

1 Institut National de la Recherche Agronomique, Université Blaise Pascal (INRA-UBP), UMR 1095, Genetics Diversity and Ecophysiology of Cereals, Clermont-Ferrand, France.
2 Institute of Evolution, University of Haifa, Haifa, Israel.
3 Department of Plant Sciences, North Dakota State University, Fargo, ND, USA.
4 Commonwealth Scientific and Industrial Research Organization Plant Industry, Canberra, Australia.
5 Agriculture and Agri-Food Canada, Cereal Research Centre, Winnipeg, Canada.
6 Diversity Arrays Technology, Yarralumla, Canberra, Australia.
7 INRA–Unité de Recherches en Génomique-Info, Versailles, France.
8 INRA–Centre National de Ressources Génomiques Végétales, Toulouse, France.
9 International Wheat Genome Sequencing Consortium, Eversole Associates, Bethesda, MD, USA.
10 Centre for Comparative Genomics, Murdoch University, Perth, Australia.
11 Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Olomouc, Czech Republic.

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Single-Feature Polymorphism Mapping in Bread Wheat.
T. W. Banks, M. C. Jordan, and D. J. Somers (2009)
The Plant Genome 2, 167-178
   Abstract »    Full Text »    PDF »
Genomic approaches for designing durum wheat ready for climate change with a focus on drought.
D. Z. Habash, Z. Kehel, and M. Nachit (2009)
J. Exp. Bot. 60, 2805-2815
   Abstract »    Full Text »    PDF »
A practical algorithm for finding maximal exact matches in large sequence datasets using sparse suffix arrays.
Z. Khan, J. S. Bloom, L. Kruglyak, and M. Singh (2009)
Bioinformatics 25, 1609-1616
   Abstract »    Full Text »    PDF »
TriFLDB: A Database of Clustered Full-Length Coding Sequences from Triticeae with Applications to Comparative Grass Genomics.
K. Mochida, T. Yoshida, T. Sakurai, Y. Ogihara, and K. Shinozaki (2009)
Plant Physiology 150, 1135-1146
   Abstract »    Full Text »    PDF »
Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.).
C. Saintenac, M. Falque, O. C. Martin, E. Paux, C. Feuillet, and P. Sourdille (2009)
Genetics 181, 393-403
   Abstract »    Full Text »    PDF »
Splendor in the Grasses.
E. A. Kellogg and C. R. Buell (2009)
Plant Physiology 149, 1-3
   Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)