Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Invitrogen

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 24 January 2008
Science 8 February 2008:
Vol. 319. no. 5864, pp. 777 - 782
DOI: 10.1126/science.1149357
Prev | Table of Contents | Next

Research Articles

Innate Immune Homeostasis by the Homeobox Gene Caudal and Commensal-Gut Mutualism in Drosophila

Ji-Hwan Ryu,1* Sung-Hee Kim,1* Hyo-Young Lee,1,2 Jin Young Bai,1 Young-Do Nam,3 Jin-Woo Bae,3 Dong Gun Lee,4 Seung Chul Shin,1,5 Eun-Mi Ha,1 Won-Jae Lee1{dagger}

Although commensalism with gut microbiota exists in all metazoans, the host factors that maintain this homeostatic relationship remain largely unknown. We show that the intestinal homeobox gene Caudal regulates the commensal-gut mutualism by repressing nuclear factor kappa B–dependent antimicrobial peptide genes. Inhibition of Caudal expression in flies via RNA interference led to overexpression of antimicrobial peptides, which in turn altered the commensal population within the intestine. In particular, the dominance of one gut microbe, Gluconobacter sp. strain EW707, eventually led to gut cell apoptosis and host mortality. However, restoration of a healthy microbiota community and normal host survival in the Caudal-RNAi flies was achieved by reintroduction of the Caudal gene. These results reveal that a specific genetic deficiency within a host can profoundly influence the gut commensal microbial community and host physiology.

1 Division of Molecular Life Science, Ewha Woman's University and National Creative Research Initiative Center for Symbiosystem, Seoul 120-750, South Korea.
2 Laboratoire de BBMI, Institut Pasteur, Paris 75724, France.
3 Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, South Korea.
4 School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701, South Korea.
5 Brain Korea 21 Program, Yonsei University College of Medicine, CPO Box 8044, Seoul 120-752, South Korea.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: lwj{at}ewha.ac.kr

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Obligate Mutualist Wigglesworthia glossinidia Influences Reproduction, Digestion, and Immunity Processes of Its Host, the Tsetse Fly.
R. Pais, C. Lohs, Y. Wu, J. Wang, and S. Aksoy (2008)
Appl. Envir. Microbiol. 74, 5965-5974
   Abstract »    Full Text »    PDF »
Commensal-dependent expression of IL-25 regulates the IL-23-IL-17 axis in the intestine.
C. Zaph, Y. Du, S. A. Saenz, M. G. Nair, J. G. Perrigoue, B. C. Taylor, A. E. Troy, D. E. Kobuley, R. A. Kastelein, D. J. Cua, et al. (2008)
J. Exp. Med. 205, 2191-2198
   Abstract »    Full Text »    PDF »
Models of infectious diseases in the fruit fly Drosophila melanogaster.
M. S. Dionne and D. S. Schneider (2008)
Dis. Model. Mech. 1, 43-49
   Abstract »    Full Text »    PDF »
Bacterial-Modulated Signaling Pathways in Gut Homeostasis.
W.-J. Lee (2008)
Science Signaling 1, pe24
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)