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Originally published in Science Express on 20 November 2007
Science 21 December 2007:
Vol. 318. no. 5858, pp. 1917 - 1920
DOI: 10.1126/science.1151526
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Reports

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Junying Yu,1,2* Maxim A. Vodyanik,2 Kim Smuga-Otto,1,2 Jessica Antosiewicz-Bourget,1,2 Jennifer L. Frane,1 Shulan Tian,3 Jeff Nie,3 Gudrun A. Jonsdottir,3 Victor Ruotti,3 Ron Stewart,3 Igor I. Slukvin,2,4 James A. Thomson1,2,5*

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.

1 Genome Center of Wisconsin, Madison, WI 53706–1580, USA.
2 Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715–1299, USA.
3 WiCell Research Institute, Madison, WI 53707–7365, USA.
4 Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.
5 Department of Anatomy, University of Wisconsin-Madison, Madison, WI 53706–1509, USA.

* To whom correspondence should be addressed. E-mail: jyu{at}primate.wisc.edu (J.Y.); thomson{at}primate.wisc.edu (J.A.T.)

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