Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 28 September 2007:
Vol. 317. no. 5846, pp. 1934 - 1938
DOI: 10.1126/science.1144874
Prev | Table of Contents | Next

Reports

The Slit Receptor EVA-1 Coactivates a SAX-3/Robo–Mediated Guidance Signal in C. elegans

Kazuko Fujisawa,1 Jeffrey L. Wrana,1,2 Joseph G. Culotti1,2*

The SAX-3/roundabout (Robo) receptor has Shiga-like toxin 1 (SLT-1)/Slit–dependent and –independent functions in guiding cell and axon migrations. We identified enhancer of ventral-axon guidance defects of unc-40 mutants (EVA-1) as a Caenorhabditis elegans transmembrane receptor for SLT-1. EVA-1 has two predicted galactose-binding ectodomains, acts cell-autonomously for SLT-1/Slit–dependent axon migration functions of SAX-3/Robo, binds to SLT-1 and SAX-3, colocalizes with SAX-3 on cells, and provides cell specificity to the activation of SAX-3 signaling by SLT-1. Double mutants of eva-1 or slt-1 with sax-3 mutations suggest that SAX-3 can (when slt-1 or eva-1 function is reduced) inhibit a parallel-acting guidance mechanism, which involves UNC-40/deleted in colorectal cancer.

1 Samuel Lunenfeld Research Institute of Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada.
2 Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.

* To whom correspondence should be addressed. E-mail: culotti{at}mshri.on.ca

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Human C21orf63 is a Heparin-binding Protein.
K. Mitsunaga, J. Harada-Itadani, T. Shikanai, H. Tateno, Y. Ikehara, J. Hirabayashi, H. Narimatsu, and T. Angata (2009)
J. Biochem. 146, 369-373
   Abstract »    Full Text »    PDF »
Pioneer longitudinal axons navigate using floor plate and Slit/Robo signals.
W. T. Farmer, A. L. Altick, H. F. Nural, J. P. Dugan, T. Kidd, F. Charron, and G. S. Mastick (2008)
Development 135, 3643-3653
   Abstract »    Full Text »    PDF »
CLEC-38, A Transmembrane Protein with C-Type Lectin-Like Domains, Negatively Regulates UNC-40-Mediated Axon Outgrowth and Promotes Presynaptic Development in Caenorhabditis elegans.
G. Kulkarni, H. Li, and W. G. Wadsworth (2008)
J. Neurosci. 28, 4541-4550
   Abstract »    Full Text »    PDF »
RPM-1, a Caenorhabditis elegans Protein That Functions in Presynaptic Differentiation, Negatively Regulates Axon Outgrowth by Controlling SAX-3/robo and UNC-5/UNC5 Activity.
H. Li, G. Kulkarni, and W. G. Wadsworth (2008)
J. Neurosci. 28, 3595-3603
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)