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Originally published in Science Express on 28 June 2007
Science 3 August 2007:
Vol. 317. no. 5838, pp. 632 - 638
DOI: 10.1126/science.1144622
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Research Articles

Genome Transplantation in Bacteria: Changing One Species to Another

Carole Lartigue, John I. Glass,* Nina Alperovich, Rembert Pieper, Prashanth P. Parmar, Clyde A. Hutchison, III, Hamilton O. Smith, J. Craig Venter

As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol–mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.

The J. Craig Venter Institute, Rockville, MD 20850, USA.

* To whom correspondence should be addressed. E-mail: jglass{at}jcvi.org

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