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Science 13 July 2007:
Vol. 317. no. 5835, pp. 242 - 245
DOI: 10.1126/science.1140649
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Reports

Postreplicative Formation of Cohesion Is Required for Repair and Induced by a Single DNA Break

Lena Ström,1 Charlotte Karlsson,1 Hanna Betts Lindroos,1 Sara Wedahl,1 Yuki Katou,2 Katsuhiko Shirahige,2 Camilla Sjögren1*

Sister-chromatid cohesion, established during replication by the protein complex cohesin, is essential for both chromosome segregation and double-strand break (DSB) repair. Normally, cohesion formation is strictly limited to the S phase of the cell cycle, but DSBs can trigger cohesion also after DNA replication has been completed. The function of this damage-induced cohesion remains unknown. In this investigation, we show that damage-induced cohesion is essential for repair in postreplicative cells in yeast. Furthermore, it is established genome-wide after induction of a single DSB, and it is controlled by the DNA damage response and cohesin-regulating factors. We thus define a cohesion establishment pathway that is independent of DNA duplication and acts together with cohesion formed during replication in sister chromatid–based DSB repair.

1 Department of Cell and Molecular Biology, Karolinska Institute, 171 77 Stockholm, Sweden.
2 Gene Research Centre, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8501 Yokohama, Japan.

* To whom correspondence should be addressed. E-mail: camilla.sjogren{at}ki.se

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The cohesin complex and its roles in chromosome biology.
J.-M. Peters, A. Tedeschi, and J. Schmitz (2008)
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Sister Chromatid Cohesion Role for CDC28-CDK in Saccharomyces cerevisiae.
A. Brands and R. V. Skibbens (2008)
Genetics 180, 7-16
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Eco1-Dependent Cohesin Acetylation During Establishment of Sister Chromatid Cohesion.
T. R. Ben-Shahar, S. Heeger, C. Lehane, P. East, H. Flynn, M. Skehel, and F. Uhlmann (2008)
Science 321, 563-566
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A Molecular Determinant for the Establishment of Sister Chromatid Cohesion.
E. Unal, J. M. Heidinger-Pauli, W. Kim, V. Guacci, I. Onn, S. P. Gygi, and D. E. Koshland (2008)
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The molecular mechanism underlying Roberts syndrome involves loss of ESCO2 acetyltransferase activity.
M. Gordillo, H. Vega, A. H. Trainer, F. Hou, N. Sakai, R. Luque, H. Kayserili, S. Basaran, F. Skovby, R. C. M. Hennekam, et al. (2008)
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Chromosome cohesion - rings, knots, orcs and fellowship.
L. A. Diaz-Martinez, J. F. Gimenez-Abian, and D. J. Clarke (2008)
J. Cell Sci. 121, 2107-2114
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DNA Double-Strand Breaks Trigger Genome-Wide Sister-Chromatid Cohesion Through Eco1 (Ctf7).
E. Unal, J. M. Heidinger-Pauli, and D. Koshland (2007)
Science 317, 245-248
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Science. ISSN 0036-8075 (print), 1095-9203 (online)