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Science 27 April 2007:
Vol. 316. no. 5824, pp. 590 - 593
DOI: 10.1126/science.1136031
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Reports

NOV (CCN3) Functions as a Regulator of Human Hematopoietic Stem or Progenitor Cells

Rajeev Gupta, Dengli Hong, Francisco Iborra, Samantha Sarno, Tariq Enver*

Clinically successful hematopoietic cell transplantation is dependent on hematopoietic stem and progenitor cells. Here we identify the matricellular protein Nephroblastoma Overexpressed (Nov, CCN3) as being essential for their functional integrity. Nov expression is restricted to the primitive (CD34) compartments of umbilical vein cord blood, and its knockdown in these cells by lentivirus-mediated RNA interference abrogates their function in vitro and in vivo. Conversely, forced expression of Nov and addition of recombinant Nov protein both enhance primitive stem and/or progenitor activity. Taken together, our results identify Nov (CCN3) as a regulator of human hematopoietic stem or progenitor cells.

Medical Research Council Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, OX3 9DS, UK.

* To whom correspondence should be addressed. E-mail: tenver{at}gwmail.jr2.ox.ac.uk

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