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Originally published in Science Express on 5 April 2007
Science 20 April 2007:
Vol. 316. no. 5823, pp. 417 - 421
DOI: 10.1126/science.1141314
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Research Articles

Genes Required for Mitotic Spindle Assembly in Drosophila S2 Cells

Gohta Goshima,1,3* Roy Wollman,2,3 Sarah S. Goodwin,1 Nan Zhang,1 Jonathan M. Scholey,2 Ronald D. Vale,1,3{dagger} Nico Stuurman1,3

The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.

1 Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
2 Department of Molecular and Cellular Biology, University of California, Davis, One Shields Avenue, 3203 Life Sciences, Davis, CA 95616, USA.
3 Physiology Course, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA.

* Present address: Institute for Advanced Research, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.

{dagger} To whom correspondence should be addressed. E-mail: vale{at}cmp.ucsf.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)