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Untemplated Oligoadenylation Promotes Degradation of RISC-Cleaved Transcripts

Fadia Ibrahim,^* Jennifer Rohr,^* Won-Joong Jeong, Jennifer Hesson, Heriberto Cerutti

Double-stranded RNA, processed to small interfering RNAs (siRNAs) by Dicer and incorporated into the RNA-induced silencing complex (RISC), triggers gene silencing by a variety of pathways in eukaryotes. RNA interference involving the degradation of homologous transcripts is the best-characterized mechanism. However, the fate of the RNA fragments resulting from siRNA-directed cleavage is poorly understood. We have identified a gene (MUT68) in the unicellular green alga Chlamydomonas reinhardtii that is required for the efficient decay of siRNA-targeted transcripts. MUT68 encodes a noncanonical polyadenylate polymerase that adds untemplated adenines to the 5' RNA fragments after siRNA-mediated cleavage and appears to stimulate their exosome-dependent degradation.

Biological Sciences and Plant Science Initiative, University of Nebraska, Lincoln, NE 68588, USA.

^* These authors contributed equally to this work.

To whom correspondence should be addressed. E-mail: hcerutti{at}unl.edu

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Degradation of a Polyadenylated rRNA Maturation By-Product Involves One of the Three RRP6-Like Proteins in Arabidopsis thaliana.

H. Lange, S. Holec, V. Cognat, L. Pieuchot, M. Le Ret, J. Canaday, and D. Gagliardi (2008)
Mol. Cell. Biol. 28, 3038-3044
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Diversification of the Core RNA Interference Machinery in Chlamydomonas reinhardtii and the Role of DCL1 in Transposon Silencing.

J. A. Casas-Mollano, J. Rohr, E.-J. Kim, E. Balassa, K. van Dijk, and H. Cerutti (2008)
Genetics 179, 69-81
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Genome-Based Analysis of Chlamydomonas reinhardtii Exoribonucleases and Poly(A) Polymerases Predicts Unexpected Organellar and Exosomal Features.

S. L. Zimmer, Z. Fei, and D. B. Stern (2008)
Genetics 179, 125-136
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Characterization of Hypovirus-Derived Small RNAs Generated in the Chestnut Blight Fungus by an Inducible DCL-2-Dependent Pathway.

X. Zhang, G. C. Segers, Q. Sun, F. Deng, and D. L. Nuss (2008)
J. Virol. 82, 2613-2619
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New ways to meet your (3') end oligouridylation as a step on the path to destruction.

C. J. Wilusz and J. Wilusz (2008)
Genes & Dev. 22, 1-7
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Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'.

T. E. Mullen and W. F. Marzluff (2008)
Genes & Dev. 22, 50-65
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3' Terminal oligo U-tract-mediated stimulation of decapping.

M.-G. Song and M. Kiledjian (2007)
RNA 13, 2356-2365
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