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Science 15 December 2006:
Vol. 314. no. 5806, p. 1653
DOI: 10.1126/science.314.5806.1653h
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This Week in Science

About 20 years ago, the development of transgenic methods that used P element transformation greatly facilitated gene analysis in Drosophila melanogaster. However, the method limits the size of DNA fragments for insertion and can only target specific sites in the genome. Venken et al. (p. 1747, published online 30 November) now develop a tool, termed P[acman], that allows site-pecific insertion of DNA fragments of more than 100 kilobases. The method will facilitate structural and functional analyses of any Drosophila gene and will permit tagging of proteins in vivo.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)