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Science 24 November 2006:
Vol. 314. no. 5803, p. 1241
DOI: 10.1126/science.314.5803.1241a
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Letters
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Technical Comment Abstracts
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COMMENT ON Papers by Chong et al., Nishio et al., and Suri et al. on Diabetes Reversal in NOD Mice
Denise L. Faustman, Simon D. Tran, Shohta Kodama, Beatrijs M. Lodde, Ildiko Szalayova, Sharon Key, Zsuzsanna Toth, Éva Mezey
Abstract: Chong et al., Nishio et al., and Suri et al. (Reports, 24 March 2006, pp. 1774, 1775, and 1778) confirmed that treating nonobese diabetic (NOD) mice with an immune adjuvant and semisyngenic spleen cells can reverse the disease but found that spleen cells did not contribute to the observed recovery of pancreatic islets. We show that islet regeneration predominately originates from endogenous cells but that introduced spleen cells can also contribute to islet recovery.
Full text at www.sciencemag.org/cgi/content/full/314/5803/1243a
RESPONSE TO COMMENTS ON Chong et al. on Diabetes Reversal in NOD Mice
Anita S. Chong, Jikun Shen, Jing Tao, Dengping Yin, Andrey Kuznetsov, Manami Hara, Louis H. Philipson
Abstract: We failed to detect transdifferentiation of spleen cells into  cells following diabetes reversal in nonobese diabetic (NOD) mice, thus contradicting a key finding of a 2003 report. We respond to Faustman et al. by justifying the use of mouse insulin promoter-green fluorescent protein transgenic mice as an appropriate system for detecting spleen-derived cells in the islets of cured NOD mice.
Full text at www.sciencemag.org/cgi/content/full/314/5803/1243b
RESPONSE TO COMMENTS ON Nishio et al. on Diabetes Reversal in NOD Mice
Junko Nishio, Jason L. Gaglia, Stuart E. Turvey, Christopher Campbell, Christophe Benoist, Diane Mathis
Abstract: Contrary to previous findings, we found no significant differentiation of splenocytes into pancreatic islet cells in nonobese diabetic (NOD) mice treated with an immune adjuvant and allogenic spleen cells. We show that our single-nucleotide polymorphism assay has the requisite sensitivity to support our contention. The experiments of Faustman et al. lack adequate controls, and we maintain that no evidence of islet regeneration has been presented.
Full text at www.sciencemag.org/cgi/content/full/314/5803/1243c
RESPONSE TO COMMENTS ON Suri et al. on Diabetes Reversal in NOD Mice
Anish Suri and Emil R. Unanue
Abstract: Faustman et al. present no new information to explain why three independent laboratories failed to reproduce their previous results implicating spleen cell transdifferentiation in the reversal of murine type 1 diabetes. Modulation of the immunological process in nonobese diabetic (NOD) mice has been accomplished by many laboratories using different protocols and does not represent a novel finding in their work.
Full text at www.sciencemag.org/cgi/content/full/314/5803/1243d
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