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Originally published in Science Express on 24 August 2006
Science 29 September 2006:
Vol. 313. no. 5795, pp. 1968 - 1972
DOI: 10.1126/science.1131981

Reports

Structure of the Exon Junction Core Complex with a Trapped DEAD-Box ATPase Bound to RNA

Christian B. F. Andersen,1,2* Lionel Ballut,1,4* Jesper S. Johansen,1,2 Hala Chamieh,4 Klaus H. Nielsen,1,2 Cristiano L. P. Oliveira,2,3 Jan Skov Pedersen,2,3 Bertrand Séraphin,4 Hervé Le Hir,4{dagger} Gregers Rom Andersen1,2{dagger}

In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.

1 Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark.
2 Centre for mRNP Biogenesis and Metabolism, University of Aarhus, DK-8000 Aarhus, Denmark.
3 Department of Chemistry and iNANO Interdisciplinary Nanoscience Center, University of Aarhus, DK-8000 Aarhus, Denmark.
4 Equipe Labélisée La Ligue, Centre de Génétique Moléculaire, associé à l'Université Paris 6, CNRS UPR2167, Avenue de la Terrasse, 91198 Gifsur-Yvette, France.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: gra{at}mb.au.dk (G.R.A.); herve.lehir{at}cgm.cnrs-gif.fr (H.L.H.).

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Science. ISSN 0036-8075 (print), 1095-9203 (online)