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Originally published in Science Express on 7 September 2006
Science 29 September 2006:
Vol. 313. no. 5795, pp. 1935 - 1942
DOI: 10.1126/science.1131127
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Research Articles

Structure of the 70S Ribosome Complexed with mRNA and tRNA

Maria Selmer,* Christine M. Dunham,* Frank V. Murphy, IV, Albert Weixlbaumer, Sabine Petry, Ann C. Kelley, John R. Weir, V. Ramakrishnan{dagger}

The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.

MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: ramak{at}mrc-lmb.cam.ac.uk

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Science. ISSN 0036-8075 (print), 1095-9203 (online)