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Science 15 September 2006:
Vol. 313. no. 5793, p. 1537
DOI: 10.1126/science.313.5793.1537n
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This Week in Science

The resolution of cellular fluorescence imaging with conventional optical techniques is limited by diffraction to a size around two orders of magnitude greater than that of most proteins. Betzig et al. (p. 1642; see the 11 August news story by Couzin) introduce a method, termed photoactivated localization microscopy (PALM), for optical imaging of intracellular fluorescent proteins at nanometer resolution. In cryo-prepared thin sections, PALM images of membrane proteins in lysosomes and the Golgi apparatus show complex ultrastructure not resolvable by total internal reflection fluorescence microscopy. PALM images have also been obtained in fixed cultured cells for cytoskeletal proteins at or near the plasma membrane.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)