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Science 21 April 2006:
Vol. 312. no. 5772, pp. 440 - 443
DOI: 10.1126/science.1124196
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Reports

Nuclear Pores Form de Novo from Both Sides of the Nuclear Envelope

Maximiliano A. D'Angelo,* Daniel J. Anderson,* Erin Richard, Martin W. Hetzer{dagger}

Nuclear pore complexes are multiprotein channels that span the double lipid bilayer of the nuclear envelope. How new pores are inserted into the intact nuclear envelope of proliferating and differentiating eukaryotic cells is unknown. We found that the Nup107-160 complex was incorporated into assembly sites in the nuclear envelope from both the nucleoplasmic and the cytoplasmic sides. Nuclear pore insertion required the generation of Ran guanosine triphosphate in the nuclear and cytoplasmic compartments. Newly formed nuclear pore complexes did not contain structural components of preexisting pores, suggesting that they can form de novo.

Salk Institute for Biological Studies, Molecular and Cell Biology Laboratory, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

* These authors contributed equally to this work

{dagger} To whom correspondence should be addressed. E-mail: hetzer{at}salk.edu

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