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Originally published in Science Express on 16 February 2006
Science 7 April 2006:
Vol. 312. no. 5770, pp. 75 - 79
DOI: 10.1126/science.1122689
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Research Articles

Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs

Antonio J. Giraldez,1,2* Yuichiro Mishima,3 Jason Rihel,1,2 Russell J. Grocock,4 Stijn Van Dongen,4 Kunio Inoue,3 Anton J. Enright,4 Alexander F. Schier1,2*

MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA molecules (mRNAs). Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.

1 Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA.
2 Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
3 Department of Biology, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodaicho, Nadaku, Kobe 657-8501, Japan.
4 Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.

* To whom correspondence should be addressed. E-mail: schier{at}fas.harvard.edu (A.F.S.); giraldez{at}mcb.harvard.edu (A.J.G.)

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Science. ISSN 0036-8075 (print), 1095-9203 (online)