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Science 7 April 2006:
Vol. 312. no. 5770, p. 55
DOI: 10.1126/science.1122000
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Technical Comments

Comment on "PDK1 Nucleates T Cell Receptor-Induced Signaling Complex for NF-{kappa}B Activation"

Thomas Gruber,1 Michael Freeley,2* Nikolaus Thuille,1 Isabelle Heit,3 Stephen Shaw,4 Aideen Long,2* Gottfried Baier1{dagger}

We observe that protein kinase C {theta} (PKC{theta}) is phosphorylated on the activation loop at threonine 538 (Thr-538) before T cell activation. Our results are inconsistent with the conclusions of Lee et al. (Reports, 1 April 2005, p. 114) that the Thr-538 phosphorylation of PKC{theta} is regulated by T cell receptor activation. Other mechanisms, such as autophosphorylation of Thr-219, might orchestrate the cellular function of PKC{theta} in T cells.

1 Department for Medical Genetics, Molecular and Clinical Pharmacology, Innsbruck Medical University, Austria.
2 Department of Biochemistry, Royal College of Surgeons, Dublin, Ireland.
3 AltanaPharma, Konstanz, Germany.
4 Experimental Immunology Branch, National Cancer Institute, Bethesda, MD 20892, USA.

* Present address: Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland.

{dagger} To whom correspondence should be addressed. E-mail: gottfried.baier{at}i-med.ac.at

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Science. ISSN 0036-8075 (print), 1095-9203 (online)