Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
This article has been retracted

Science 17 February 2006:
Vol. 311. no. 5763, pp. 992 - 996
DOI: 10.1126/science.1120925
Prev | Table of Contents | Next

Reports

Cdx2 Gene Expression and Trophectoderm Lineage Specification in Mouse Embryos

Kaushik Deb,1 Mayandi Sivaguru,2 Hwan Yul Yong,1 R. Michael Roberts1,3*

Controversy exists as to whether individual blastomeres from two-cell-stage mouse embryos have identical developmental properties and fate. We show that the transcription factor Cdx2 is expressed in the nuclei of cells derived from the late-dividing but not the first-dividing blastomere of two-cell embryos and, by lineage tracing and RNA interference knock-down experiments, that this lagging cell is the precursor of trophectoderm. Cdx2 mRNA is localized toward the vegetal pole of oocytes, reorients after fertilization, and becomes concentrated in the late-dividing, two-cell-stage blastomere. The asymmetrical distribution of Cdx2 gene products in the oocyte and embryo defines the lineage to trophectoderm.

1 Department of Animal Sciences, Columbia, MO 65211, USA.
2 Department of Biological Sciences, Columbia, MO 65211, USA.
3 Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA.

* To whom correspondence should be addressed. E-mail: robertsrm{at}missouri.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Polony analysis of gene expression in ES cells and blastocysts.
C. Rieger, R. Poppino, R. Sheridan, K. Moley, R. Mitra, and D. Gottlieb (2007)
Nucleic Acids Res.
   Abstract »    Full Text »    PDF »
Stochastic patterning in the mouse pre-implantation embryo.
J.-E. Dietrich and T. Hiiragi (2007)
Development 134, 4219-4231
   Abstract »    Full Text »    PDF »
Transcription factor TEAD4 specifies the trophectoderm lineage at the beginning of mammalian development.
R. Yagi, M. J. Kohn, I. Karavanova, K. J. Kaneko, D. Vullhorst, M. L. DePamphilis, and A. Buonanno (2007)
Development 134, 3827-3836
   Abstract »    Full Text »    PDF »
Reprogramming somatic cells into stem cells..
R. Alberio, K. H Campbell, and A. D Johnson (2006)
Reproduction 132, 709-720
   Abstract »    Full Text »    PDF »
Escaping the tyranny of the embryo? A new approach to ART regulation based on UK and Australian experiences.
M. H. Johnson (2006)
Hum. Reprod. 21, 2756-2765
   Abstract »    Full Text »    PDF »
Allocation of Cells in Mouse Blastocyst Is Not Determined by the Order of Cleavage of the First Two Blastomeres.
M. Waksmundzka, A. Wisniewska, and M. Maleszewski (2006)
Biol Reprod 75, 582-587
   Abstract »    Full Text »    PDF »
Geminin is essential to prevent endoreduplication and to form pluripotent cells during mammalian development.
M. A. Gonzalez, K.-e K. Tachibana, D. J. Adams, L. van der Weyden, M. Hemberger, N. Coleman, A. Bradley, and R. A. Laskey (2006)
Genes & Dev. 20, 1880-1884
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)