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Science 28 October 2005:
Vol. 310. no. 5748, pp. 674 - 676
DOI: 10.1126/science.1118476
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Reports

The V-Antigen of Yersinia Forms a Distinct Structure at the Tip of Injectisome Needles

Catherine A. Mueller,1* Petr Broz,1* Shirley A. Müller,1,2 Philippe Ringler,1,2 Françoise Erne-Brand,1,2 Isabel Sorg,1 Marina Kuhn,1 Andreas Engel,1,2 Guy R. Cornelis1{dagger}

Many pathogenic bacteria use injectisomes to deliver effector proteins into host cells through type III secretion. Injectisomes consist of a basal body embedded in the bacterial membranes and a needle. In Yersinia, translocation of effectors requires the YopB and YopD proteins, which form a pore in the target cell membrane, and the LcrV protein, which assists the assembly of the pore. Here we report that LcrV forms a distinct structure at the tip of the needle, the tip complex. This unique localization of LcrV may explain its crucial role in the translocation process and its efficacy as the main protective antigen against plague.

1 Biozentrum der Universität Basel, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland.
2 Maurice E. Müller Institute, Klingelbergstrasse 50-70, CH-4056, Basel, Switzerland.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: guy.cornelis{at}unibas.ch

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