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Science 2 September 2005:
Vol. 309. no. 5740, pp. 1581 - 1584
DOI: 10.1126/science.1116102
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Reports

Recombination Regulation by Transcription-Induced Cohesin Dissociation in rDNA Repeats

Takehiko Kobayashi1,2* and Austen R. D. Ganley1

Organisms maintain ribosomal RNA gene repeats (rDNA) at stable copy numbers by recombination; the loss of repeats results in gene amplification. Here we report a mechanism of amplification regulation. We show that amplification is dependent on transcription from a noncoding bidirectional promoter (E-pro) within the rDNA spacer. E-pro transcription stimulates the dissociation of cohesin, a DNA binding protein complex that suppresses sister-chromatid-based changes in rDNA copy number. This transcription is regulated by the silencing gene, SIR2, and by copy number. Transcription-induced cohesin dissociation may be a general mechanism of recombination regulation.

1 National Institute for Basic Biology, SOKENDAI, School of Life Science, 38 Nishigonaka, Myodaijicho, Okazaki, 444-8585 Japan.
2 The Graduate University for Advanced Studies, SOKENDAI, School of Life Science, 38 Nishigonaka, Myodaijicho, Okazaki, 444-8585 Japan.

* To whom correspondence should be addressed. E-mail: koba{at}nibb.ac.jp

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Science. ISSN 0036-8075 (print), 1095-9203 (online)