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Recombination Regulation by Transcription-Induced Cohesin Dissociation in rDNA Repeats
Takehiko Kobayashi1,2* and
Austen R. D. Ganley1
Organisms maintain ribosomal RNA gene repeats (rDNA) at stablecopy numbers by recombination; the loss of repeats results ingene amplification. Here we report a mechanism of amplificationregulation. We show that amplification is dependent on transcriptionfrom a noncoding bidirectional promoter (E-pro) within the rDNAspacer. E-pro transcription stimulates the dissociation of cohesin,a DNA binding protein complex that suppresses sister-chromatid-basedchanges in rDNA copy number. This transcription is regulatedby the silencing gene, SIR2, and by copy number. Transcription-inducedcohesin dissociation may be a general mechanism of recombinationregulation.
1 National Institute for Basic Biology, SOKENDAI, School of Life Science, 38 Nishigonaka, Myodaijicho, Okazaki, 444-8585 Japan. 2 The Graduate University for Advanced Studies, SOKENDAI, School of Life Science, 38 Nishigonaka, Myodaijicho, Okazaki, 444-8585 Japan.
* To whom correspondence should be addressed. E-mail: koba{at}nibb.ac.jp
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