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Science 12 August 2005:
Vol. 309. no. 5737, pp. 1088 - 1090
DOI: 10.1126/science.1113435
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Reports

In Situ Stable Isotope Probing of Methanogenic Archaea in the Rice Rhizosphere

Yahai Lu1,2 and Ralf Conrad2*

Microorganisms living in anoxic rice soils contribute 10 to 25% of global methane emissions. The most important carbon source for CH4 production is plant-derived carbon that enters soil as root exudates and debris. Pulse labeling of rice plants with 13CO2 resulted in incorporation of 13C into the ribosomal RNA of Rice Cluster I Archaea in the soil, indicating that this archaeal group plays a key role in CH4 production from plant-derived carbon. This group of microorganisms has not yet been isolated but appears to be of global environmental importance.

1 College of Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China.
2 Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße, 35043 Marburg, Germany.

* To whom correspondence should be addressed. E-mail: conrad{at}staff.uni-marburg.de

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