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Complete Replication of Hepatitis C Virus in Cell Culture
Brett D. Lindenbach,1Matthew J. Evans,1Andrew J. Syder,1Benno Wölk,1Timothy L. Tellinghuisen,1Christopher C. Liu,2Toshiaki Maruyama,3*Richard O. Hynes,2Dennis R. Burton,3Jane A. McKeating,1Charles M. Rice1
Many aspects of the hepatitis C virus (HCV) life cycle havenot been reproduced in cell culture, which has slowed researchprogress on this important human pathogen. Here, we describea full-length HCV genome that replicates and produces virusparticles that are infectious in cell culture (HCVcc). Replicationof HCVcc was robust, producing nearly 105 infectious units permilliliter within 48 hours. Virus particles were filterableand neutralized with a monoclonal antibody against the viralglycoprotein E2. Viral entry was dependent on cellular expressionof a putative HCV receptor, CD81. HCVcc replication was inhibitedby interferon- and by several HCV-specific antiviral compounds,suggesting that this in vitro system will aid in the searchfor improved antivirals.
1 Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. 2 Howard Hughes Medical Institute, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. 3 Departments of Immunology and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Published online 9 June 2005
Include this information when citing this paper.
* Present address: Alexion Antibody Technologies, San Diego, CA92121, USA.
Present address: Division of Immunity and Infection, Instituteof Biomedical Research, University of Birmingham Medical School,Birmingham B15 2TT, UK.
To whom correspondence should be addressed. E-mail: ricec{at}rockefeller.edu
The editors suggest the following Related Resources on Science sites:
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|Abstract »|Full Text »
and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals. 
Present address: Division of Immunity and Infection, Institute of Biomedical Research, University of Birmingham Medical School, Birmingham B15 2TT, UK. 
To whom correspondence should be addressed. E-mail: