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Science 20 May 2005:
Vol. 308. no. 5725, pp. 1174 - 1178
DOI: 10.1126/science.1110225
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Reports

The Intracellular Fate of Salmonella Depends on the Recruitment of Kinesin

Emmanuel Boucrot,1 Thomas Henry,1 Jean-Paul Borg,2 Jean-Pierre Gorvel,1 Stéphane Méresse1*

Salmonella enterica causes a variety of diseases, including gastroenteritis and typhoid fever. The success of this pathogen depends on its capacity to proliferate within host cells in a membrane-bound compartment. We found that the Salmonella-containing vacuole recruited the plus-end–directed motor kinesin. Bacterial effector proteins translocated into the host cell by a type III secretion system antagonistically regulated this event. Among these effectors, SifA targeted SKIP, a host protein that down-regulated the recruitment of kinesin on the bacterial vacuole and, in turn, controlled vacuolar membrane dynamics.

1 Centre d'Immunologie de Marseille-Luminy, CNRS-INSERM-Université de la Méditerranée, Parc Scientifique de Luminy, Case 906-13288 Marseille Cedex 9, France.
2 Molecular Pharmacology, UMR 599 INSERM, and Institut Paoli-Calmettes, 27 Boulevard Leï Roure, 13009 Marseille, France.

* To whom correspondence should be addressed. E-mail: meresse{at}ciml.univ-mrs.fr

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