Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 7 January 2005:
Vol. 307. no. 5706, pp. 113 - 117
DOI: 10.1126/science.1105143
Prev | Table of Contents | Next

Reports

Disulfide Isomerization After Membrane Release of Its SAR Domain Activates P1 Lysozyme

Min Xu,* Arockiasamy Arulandu,* Douglas K. Struck, Stephanie Swanson, James C. Sacchettini,{dagger} Ry Young{dagger}

The P1 lysozyme Lyz is secreted to the periplasm of Escherichia coli and accumulates in an inactive membrane-tethered form. Genetic and biochemical experiments show that, when released from the bilayer, Lyz is activated by an intramolecular thiol-disulfide isomerization, which requires a cysteine in its N-terminal SAR (signal-arrest-release) domain. Crystal structures confirm the alternative disulfide linkages in the two forms of Lyz and reveal dramatic conformational differences in the catalytic domain. Thus, the exported P1 endolysin is kept inactive by three levels of control—topological, conformational, and covalent—until its release from the membrane is triggered by the P1 holin.

Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843–2128, USA.



* These authors contributed equally to the results described in this work.

{dagger} To whom correspondence should be addressed. E-mail: sacchett{at}tamu.edu (J.C.S.) and ryland{at}tamu.edu (R.Y.).

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Genomic and Biological Analysis of Phage Xfas53 and Related Prophages of Xylella fastidiosa.
E. J. Summer, C. J. Enderle, S. J. Ahern, J. J. Gill, C. P. Torres, D. N. Appel, M. C. Black, R. Young, and C. F. Gonzalez (2010)
J. Bacteriol. 192, 179-190
   Abstract »    Full Text »    PDF »
Structure of the lethal phage pinhole.
T. Pang, C. G. Savva, K. G. Fleming, D. K. Struck, and R. Young (2009)
PNAS 106, 18966-18971
   Abstract »    Full Text »    PDF »
The Autolysin LytA Contributes to Efficient Bacteriophage Progeny Release in Streptococcus pneumoniae.
M. J. Frias, J. Melo-Cristino, and M. Ramirez (2009)
J. Bacteriol. 191, 5428-5440
   Abstract »    Full Text »    PDF »
Layers of Evolvability in a Bacteriophage Life History Trait.
R. H. Heineman, J. J. Bull, and I. J. Molineux (2009)
Mol. Biol. Evol. 26, 1289-1298
   Abstract »    Full Text »    PDF »
Evolutionary dominance of holin lysis systems derives from superior genetic malleability.
Y. Zheng, D. K. Struck, C. A. Dankenbring, and R. Young (2008)
Microbiology 154, 1710-1718
   Abstract »    Full Text »    PDF »
Molecular Control of Bacterial Death and Lysis.
K. C. Rice and K. W. Bayles (2008)
Microbiol. Mol. Biol. Rev. 72, 85-109
   Abstract »    Full Text »    PDF »
The Pinholin of Lambdoid Phage 21: Control of Lysis by Membrane Depolarization.
T. Park, D. K. Struck, C. A. Dankenbring, and R. Young (2007)
J. Bacteriol. 189, 9135-9139
   Abstract »    Full Text »    PDF »
The T4 RI Antiholin Has an N-Terminal Signal Anchor Release Domain That Targets It for Degradation by DegP.
T. A. T. Tran, D. K. Struck, and R. Young (2007)
J. Bacteriol. 189, 7618-7625
   Abstract »    Full Text »    PDF »
Topological dynamics of holins in programmed bacterial lysis.
T. Park, D. K. Struck, J. F. Deaton, and R. Young (2006)
PNAS 103, 19713-19718
   Abstract »    Full Text »    PDF »
Genomic Analysis of Pseudomonas aeruginosa Phages LKD16 and LKA1: Establishment of the {phi}KMV Subgroup within the T7 Supergroup..
P.-J. Ceyssens, R. Lavigne, W. Mattheus, A. Chibeu, K. Hertveldt, J. Mast, J. Robben, and G. Volckaert (2006)
J. Bacteriol. 188, 6924-6931
   Abstract »    Full Text »    PDF »
Bacteriophage endolysins as a novel class of antibacterial agents..
J. Borysowski, B. Weber-Dabrowska, and A. Gorski (2006)
Experimental Biology and Medicine 231, 366-377
   Abstract »    Full Text »    PDF »
Divergence and Mosaicism among Virulent Soil Phages of the Burkholderia cepacia Complex.
E. J. Summer, C. F. Gonzalez, M. Bomer, T. Carlile, A. Embry, A. M. Kucherka, J. Lee, L. Mebane, W. C. Morrison, L. Mark, et al. (2006)
J. Bacteriol. 188, 255-268
   Abstract »    Full Text »    PDF »
Lysis Timing and Bacteriophage Fitness.
I.-N. Wang (2006)
Genetics 172, 17-26
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)