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Science 5 November 2004:
Vol. 306. no. 5698, p. 997
DOI: 10.1126/science.1103521
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Uridine Addition After MicroRNA-Directed Cleavage

Binzhang Shen and Howard M. Goodman*

One of the important roles of microRNA (miRNA) is to direct the cleavage of messenger RNA (mRNA). However, the mechanisms of decay of the cleaved mRNA products is not well understood. We show that miRNA-directed cleavage products in organisms as diverse as Arabidopsis, mouse, and Epstein-Barr virus have at their 3' ends a stretch (1 to 24 nucleotides) of oligouridine posttranscriptionally added downstream of the cleavage site. This 3' uridine addition, as shown for Arabidopsis, is correlated with decapping and 5' shortening of the cleaved products, suggesting a mechanistic step in the miRNA-directed mRNA decay mechanism.

Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, 50 Blossom Street, Boston, MA 02114, USA.

* To whom correspondence should be addressed. E-mail: goodman{at}molbio.mgh.harvard.edu

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