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Originally published in Science Express on 30 September 2004
Science 5 November 2004:
Vol. 306. no. 5698, pp. 1025 - 1028
DOI: 10.1126/science.1103185
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Reports

Accumulation of Mn(II) in Deinococcus radiodurans Facilitates Gamma-Radiation Resistance

M. J. Daly,1* E. K. Gaidamakova,1 V. Y. Matrosova,1 A. Vasilenko,1 M. Zhai,1 A. Venkateswaran,1 M. Hess,1 M. V. Omelchenko,1,2 H. M. Kostandarithes,3 K. S. Makarova,2 L. P. Wackett,4 J. K. Fredrickson,3 D. Ghosal1

Deinococcus radiodurans is extremely resistant to ionizing radiation. How this bacterium can grow under chronic {gamma} radiation [50 grays (Gy) per hour] or recover from acute doses greater than 10 kGy is unknown. We show that D. radiodurans accumulates very high intracellular manganese and low iron levels compared with radiation-sensitive bacteria and that resistance exhibits a concentration-dependent response to manganous chloride [Mn(II)]. Among the most radiation-resistant bacterial groups reported, Deinococcus, Enterococcus, Lactobacillus, and cyanobacteria accumulate Mn(II). In contrast, Shewanella oneidensis and Pseudomonas putida have high iron but low intracellular manganese concentrations and are very sensitive. We propose that Mn(II) accumulation facilitates recovery from radiation injury.

1 Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
2 National Institutes of Health, Bethesda, MD 20894, USA.
3 Pacific Northwest National Laboratory, Richland, WA 99352, USA.
4 University of Minnesota, St. Paul, MN 55108, USA.

* To whom correspondence should be addressed. E-mail: mdaly{at}usuhs.mil

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Science. ISSN 0036-8075 (print), 1095-9203 (online)