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Science 22 October 2004:
Vol. 306. no. 5696, pp. 702 - 704
DOI: 10.1126/science.1102331
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Reports

Plant Cuticular Lipid Export Requires an ABC Transporter

Jamie A. Pighin,1* Huanquan Zheng,1* Laura J. Balakshin,1 Ian P. Goodman,1 Tamara L. Western,1 Reinhard Jetter,1,2 Ljerka Kunst,1 A. Lacey Samuels1{dagger}

A waxy protective cuticle coats all primary aerial plant tissues. Its synthesis requires extensive export of lipids from epidermal cells to the plant surface. Arabidopsis cer5 mutants had reduced stem cuticular wax loads and accumulated sheetlike inclusions in the cytoplasm of wax-secreting cells. These inclusions represented abnormal deposits of cuticular wax and resembled inclusions found in a human disorder caused by a defective peroxisomal adenosine triphosphate binding cassette (ABC) transporter. We found that the CER5 gene encodes an ABC transporter localized in the plasma membrane of epidermal cells and conclude that it is required for wax export to the cuticle.

1 Department of Botany, University of British Columbia (UBC), 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada.
2 Department of Chemistry, UBC, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada.



* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: lsamuels{at}interchange.ubc.ca

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