Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 22 October 2004:
Vol. 306. no. 5696, pp. 702 - 704
DOI: 10.1126/science.1102331
Prev | Table of Contents | Next

Reports

Plant Cuticular Lipid Export Requires an ABC Transporter

Jamie A. Pighin,1* Huanquan Zheng,1* Laura J. Balakshin,1 Ian P. Goodman,1 Tamara L. Western,1 Reinhard Jetter,1,2 Ljerka Kunst,1 A. Lacey Samuels1{dagger}

A waxy protective cuticle coats all primary aerial plant tissues. Its synthesis requires extensive export of lipids from epidermal cells to the plant surface. Arabidopsis cer5 mutants had reduced stem cuticular wax loads and accumulated sheetlike inclusions in the cytoplasm of wax-secreting cells. These inclusions represented abnormal deposits of cuticular wax and resembled inclusions found in a human disorder caused by a defective peroxisomal adenosine triphosphate binding cassette (ABC) transporter. We found that the CER5 gene encodes an ABC transporter localized in the plasma membrane of epidermal cells and conclude that it is required for wax export to the cuticle.

1 Department of Botany, University of British Columbia (UBC), 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada.
2 Department of Chemistry, UBC, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada.



* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: lsamuels{at}interchange.ubc.ca

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Disruption of Glycosylphosphatidylinositol-Anchored Lipid Transfer Protein Gene Altered Cuticular Lipid Composition, Increased Plastoglobules, and Enhanced Susceptibility to Infection by the Fungal Pathogen Alternaria brassicicola.
S. B. Lee, Y. S. Go, H.-J. Bae, J. H. Park, S. H. Cho, H. J. Cho, D. S. Lee, O. K. Park, I. Hwang, and M. C. Suh (2009)
Plant Physiology 150, 42-54
   Abstract »    Full Text »    PDF »
Arabidopsis LTPG Is a Glycosylphosphatidylinositol-Anchored Lipid Transfer Protein Required for Export of Lipids to the Plant Surface.
A. DeBono, T. H. Yeats, J. K.C. Rose, D. Bird, R. Jetter, L. Kunst, and L. Samuels (2009)
PLANT CELL 21, 1230-1238
   Abstract »    Full Text »    PDF »
Interaction between Arabidopsis Ca2+/H+ Exchangers CAX1 and CAX3.
J. Zhao, T. Shigaki, H. Mei, Y.-q. Guo, N.-H. Cheng, and K. D. Hirschi (2009)
J. Biol. Chem. 284, 4605-4615
   Abstract »    Full Text »    PDF »
Root System Architecture in Arabidopsis Grown in Culture Is Regulated by Sucrose Uptake in the Aerial Tissues.
D. R. MacGregor, K. I. Deak, P. A. Ingram, and J. E. Malamy (2008)
PLANT CELL 20, 2643-2660
   Abstract »    Full Text »    PDF »
Identification of the Wax Ester Synthase/Acyl-Coenzyme A:Diacylglycerol Acyltransferase WSD1 Required for Stem Wax Ester Biosynthesis in Arabidopsis.
F. Li, X. Wu, P. Lam, D. Bird, H. Zheng, L. Samuels, R. Jetter, and L. Kunst (2008)
Plant Physiology 148, 97-107
   Abstract »    Full Text »    PDF »
Tracking Monolignols during Wood Development in Lodgepole Pine.
M. Kaneda, K. H. Rensing, J. C.T. Wong, B. Banno, S. D. Mansfield, and A. L. Samuels (2008)
Plant Physiology 147, 1750-1760
   Abstract »    Full Text »    PDF »
Tapetum Degeneration Retardation is Critical for Aliphatic Metabolism and Gene Regulation during Rice Pollen Development.
D.-S. Zhang, W.-Q. Liang, Z. Yuan, N. Li, J. Shi, J. Wang, Y.-M. Liu, W.-J. Yu, and D.-B. Zhang (2008)
Mol Plant 1, 599-610
   Abstract »    Full Text »    PDF »
Gene Expression and Metabolism in Tomato Fruit Surface Tissues.
S. Mintz-Oron, T. Mandel, I. Rogachev, L. Feldberg, O. Lotan, M. Yativ, Z. Wang, R. Jetter, I. Venger, A. Adato, et al. (2008)
Plant Physiology 147, 823-851
   Abstract »    Full Text »    PDF »
Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway.
S. Taketa, S. Amano, Y. Tsujino, T. Sato, D. Saisho, K. Kakeda, M. Nomura, T. Suzuki, T. Matsumoto, K. Sato, et al. (2008)
PNAS 105, 4062-4067
   Abstract »    Full Text »    PDF »
An ABC Transporter Gene of Arabidopsis thaliana, AtWBC11, is Involved in Cuticle Development and Prevention of Organ Fusion.
B. Luo, X.-Y. Xue, W.-L. Hu, L.-J. Wang, and X.-Y. Chen (2007)
Plant Cell Physiol. 48, 1790-1802
   Abstract »    Full Text »    PDF »
P-Glycoprotein4 Displays Auxin Efflux Transporter-Like Action in Arabidopsis Root Hair Cells and Tobacco Cells.
M. Cho, S. H. Lee, and H.-T. Cho (2007)
PLANT CELL 19, 3930-3943
   Abstract »    Full Text »    PDF »
The Arabidopsis DESPERADO/AtWBC11 Transporter Is Required for Cutin and Wax Secretion.
D. Panikashvili, S. Savaldi-Goldstein, T. Mandel, T. Yifhar, R. B. Franke, R. Hofer, L. Schreiber, J. Chory, and A. Aharoni (2007)
Plant Physiology 145, 1345-1360
   Abstract »    Full Text »    PDF »
Cytological and Biochemical Analysis of COF1, an Arabidopsis Mutant of an ABC Transporter Gene.
H. Ukitsu, T. Kuromori, K. Toyooka, Y. Goto, K. Matsuoka, E. Sakuradani, S. Shimizu, A. Kamiya, Y. Imura, M. Yuguchi, et al. (2007)
Plant Cell Physiol. 48, 1524-1533
   Abstract »    Full Text »    PDF »
The Cytochrome P450 Enzyme CYP96A15 Is the Midchain Alkane Hydroxylase Responsible for Formation of Secondary Alcohols and Ketones in Stem Cuticular Wax of Arabidopsis.
S. Greer, M. Wen, D. Bird, X. Wu, L. Samuels, L. Kunst, and R. Jetter (2007)
Plant Physiology 145, 653-667
   Abstract »    Full Text »    PDF »
The Developmental Pattern of Tomato Fruit Wax Accumulation and Its Impact on Cuticular Transpiration Barrier Properties: Effects of a Deficiency in a beta-Ketoacyl-Coenzyme A Synthase (LeCER6).
J. Leide, U. Hildebrandt, K. Reussing, M. Riederer, and G. Vogg (2007)
Plant Physiology 144, 1667-1679
   Abstract »    Full Text »    PDF »
A Genomic Approach to Suberin Biosynthesis and Cork Differentiation.
M. Soler, O. Serra, M. Molinas, G. Huguet, S. Fluch, and M. Figueras (2007)
Plant Physiology 144, 419-431
   Abstract »    Full Text »    PDF »
The Transcription Factor WIN1/SHN1 Regulates Cutin Biosynthesis in Arabidopsis thaliana.
R. Kannangara, C. Branigan, Y. Liu, T. Penfield, V. Rao, G. Mouille, H. Hofte, M. Pauly, J. L. Riechmann, and P. Broun (2007)
PLANT CELL 19, 1278-1294
   Abstract »    Full Text »    PDF »
A Core Subunit of the RNA-Processing/Degrading Exosome Specifically Influences Cuticular Wax Biosynthesis in Arabidopsis.
T. S. Hooker, P. Lam, H. Zheng, and L. Kunst (2007)
PLANT CELL 19, 904-913
   Abstract »    Full Text »    PDF »
Tapetosomes in Brassica Tapetum Accumulate Endoplasmic Reticulum-Derived Flavonoids and Alkanes for Delivery to the Pollen Surface.
K. Hsieh and A. H.C. Huang (2007)
PLANT CELL 19, 582-596
   Abstract »    Full Text »    PDF »
Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development.
K.-H. Jung, M.-J. Han, D.-y. Lee, Y.-S. Lee, L. Schreiber, R. Franke, A. Faust, A. Yephremov, H. Saedler, Y.-W. Kim, et al. (2006)
PLANT CELL 18, 3015-3032
   Abstract »    Full Text »    PDF »
CER4 Encodes an Alcohol-Forming Fatty Acyl-Coenzyme A Reductase Involved in Cuticular Wax Production in Arabidopsis.
O. Rowland, H. Zheng, S. R. Hepworth, P. Lam, R. Jetter, and L. Kunst (2006)
Plant Physiology 142, 866-877
   Abstract »    Full Text »    PDF »
Leaves in turbidite sands: The main source of oil and gas in the deep-water Kutei Basin, Indonesia.
A. Saller, R. Lin, and J. Dunham (2006)
AAPG Bulletin 90, 1585-1608
   Abstract »    Full Text »    PDF »
Isolation of a novel ABC-transporter gene from soybean induced by salicylic acid.
H. Eichhorn, M. Klinghammer, P. Becht, and R. Tenhaken (2006)
J. Exp. Bot. 57, 2193-2201
   Abstract »    Full Text »    PDF »
Arabidopsis PEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration.
M. Stein, J. Dittgen, C. Sanchez-Rodriguez, B.-H. Hou, A. Molina, P. Schulze-Lefert, V. Lipka, and S. Somerville (2006)
PLANT CELL 18, 731-746
   Abstract »    Full Text »    PDF »
Genome-wide analysis of ATP-binding cassette (ABC) proteins in a model legume plant, Lotus japonicus: comparison with Arabidopsis ABC protein family.
A. Sugiyama, N. Shitan, S. Sato, Y. Nakamura, S. Tabata, and K. Yazaki (2006)
DNA Res 13, 205-228
   Abstract »    Full Text »    PDF »
Cuticular Lipid Composition, Surface Structure, and Gene Expression in Arabidopsis Stem Epidermis.
M. C. Suh, A. L. Samuels, R. Jetter, L. Kunst, M. Pollard, J. Ohlrogge, and F. Beisson (2005)
Plant Physiology 139, 1649-1665
   Abstract »    Full Text »    PDF »
Biology of a Novel Organic Solute and Steroid Transporter, OST{alpha}-OST{beta}.
N. Ballatori (2005)
Experimental Biology and Medicine 230, 689-698
   Abstract »    Full Text »    PDF »
PGP4, an ATP Binding Cassette P-Glycoprotein, Catalyzes Auxin Transport in Arabidopsis thaliana Roots.
K. Terasaka, J. J. Blakeslee, B. Titapiwatanakun, W. A. Peer, A. Bandyopadhyay, S. N. Makam, O. R. Lee, E. L. Richards, A. S. Murphy, F. Sato, et al. (2005)
PLANT CELL 17, 2922-2939
   Abstract »    Full Text »    PDF »
NpPDR1, a Pleiotropic Drug Resistance-Type ATP-Binding Cassette Transporter from Nicotiana plumbaginifolia, Plays a Major Role in Plant Pathogen Defense.
Y. Stukkens, A. Bultreys, S. Grec, T. Trombik, D. Vanham, and M. Boutry (2005)
Plant Physiology 139, 341-352
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)