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Science 22 October 2004:
Vol. 306. no. 5696, pp. 655 - 660
DOI: 10.1126/science.1101312
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Research Articles

A Gene Expression Map for the Euchromatic Genome of Drosophila melanogaster

Viktor Stolc,1,5* Zareen Gauhar,1,2* Christopher Mason,2* Gabor Halasz,7 Marinus F. van Batenburg,7,9 Scott A. Rifkin,2,3 Sujun Hua,2 Tine Herreman,2 Waraporn Tongprasit,6 Paolo Emilio Barbano,2,4 Harmen J. Bussemaker,7,8 Kevin P. White2,3{dagger}

We used a maskless photolithography method to produce DNA oligonucleotide microarrays with unique probe sequences tiled throughout the genome of Drosophila melanogaster and across predicted splice junctions. RNA expression of protein coding and nonprotein coding sequences was determined for each major stage of the life cycle, including adult males and females. We detected transcriptional activity for 93% of annotated genes and RNA expression for 41% of the probes in intronic and intergenic sequences. Comparison to genome-wide RNA interference data and to gene annotations revealed distinguishable levels of expression for different classes of genes and higher levels of expression for genes with essential cellular functions. Differential splicing was observed in about 40% of predicted genes, and 5440 previously unknown splice forms were detected. Genes within conserved regions of synteny with D. pseudoobscura had highly correlated expression; these regions ranged in length from 10 to 900 kilobase pairs. The expressed intergenic and intronic sequences are more likely to be evolutionarily conserved than nonexpressed ones, and about 15% of them appear to be developmentally regulated. Our results provide a draft expression map for the entire nonrepetitive genome, which reveals a much more extensive and diverse set of expressed sequences than was previously predicted.

1 Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
2 Department of Genetics, Yale University, New Haven, CT 06520, USA.
3 Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06520, USA.
4 Department of Mathematics, Yale University, New Haven, CT 06520, USA.
5 Genome Research Facility, NASA Ames Research Center, Mail Stop 239-11, Moffett Field, CA 94035, USA.
6 Eloret Corporation, Sunnyvale, CA 94087, USA.
7 Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
8 Center for Computational Biology and Bioinformatics, Columbia University, New York, NY 10027, USA.
9 Bioinformatics Laboratory, Academic Medical Center, University of Amsterdam, 1100 DE Amsterdam, Netherlands.



* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: kevin.white{at}yale.edu

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