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Plants with a winter growth habit flower earlier when exposedfor several weeks to cold temperatures, a process called vernalization.We report here the positional cloning of the wheat vernalizationgene VRN2, a dominant repressor of flowering that is down-regulatedby vernalization. Loss of function of VRN2, whether by naturalmutations or deletions, resulted in spring lines, which do notrequire vernalization to flower. Reduction of the RNA levelof VRN2 by RNA interference accelerated the flowering time oftransgenic winter-wheat plants by more than a month.
1 Department of Agronomy and Range Science, University of California, Davis, CA 95616, USA. 2 U.S. Department of AgricultureAgricultural Research Service, Albany, CA 94710, USA. 3 Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA. 4 Purdue University Genomics Core, Purdue University, West Lafayette, IN 47907, USA. 5 Department of Genetics, University of Georgia, Athens, GA 30602, USA.
* These authors contributed equally to this work.
Present address: Instituto Recursos Biológicos, InstitutoNacional de Tecnologia Agropecuaria, (1712) Castelar, BuenosAires, Argentina.
Present address: Consejo Nacional Investigaciones Cientificasy Tecnicas Departamento de Agronomía, Universidad Nacionaldel Sur, 8000 Bahía Blanca, Argentina.
To whom correspondence should be addressed. E-mail: jdubcovsky{at}ucdavis.edu
Involvement of the MADS-Box Gene ZMM4 in Floral Induction and Inflorescence Development in Maize.
O. N. Danilevskaya, X. Meng, D. A. Selinger, S. Deschamps, P. Hermon, G. Vansant, R. Gupta, E. V. Ananiev, and M. G. Muszynski (2008)
Plant Physiology
147, 2054-2069
|Abstract »|Full Text »|PDF »
Effects of photo and thermo cycles on flowering time in barley: a genetical phenomics approach.
I. Karsai, P. Szucs, B. Koszegi, P.M. Hayes, A. Casas, Z. Bedo, and O. Veisz (2008)
J. Exp. Bot.
59, 2707-2715
|Abstract »|Full Text »|PDF »
Molecular Plant Breeding as the Foundation for 21st Century Crop Improvement.
S. P. Moose and R. H. Mumm (2008)
Plant Physiology
147, 969-977
|Full Text »|PDF »
Low-Temperature and Daylength Cues Are Integrated to Regulate FLOWERING LOCUS T in Barley.
M. N. Hemming, W. J. Peacock, E. S. Dennis, and B. Trevaskis (2008)
Plant Physiology
147, 355-366
|Abstract »|Full Text »|PDF »
Overexpression of TaVRN1 in Arabidopsis Promotes Early Flowering and Alters Development.
H. Adam, F. Ouellet, N. A. Kane, Z. Agharbaoui, G. Major, Y. Tominaga, and F. Sarhan (2007)
Plant Cell Physiol.
48, 1192-1206
|Abstract »|Full Text »|PDF »
Genome Plasticity a Key Factor in the Success of Polyploid Wheat Under Domestication.
Evolutionary Conservation of the FLOWERING LOCUS C-Mediated Vernalization Response: Evidence From the Sugar Beet (Beta vulgaris).
P. A. Reeves, Y. He, R. J. Schmitz, R. M. Amasino, L. W. Panella, and C. M. Richards (2007)
Genetics
176, 295-307
|Abstract »|Full Text »|PDF »
Control of flowering time in temperate cereals: genes, domestication, and sustainable productivity.
J. Cockram, H. Jones, F. J. Leigh, D. O'Sullivan, W. Powell, D. A. Laurie, and A. J. Greenland (2007)
J. Exp. Bot.
|Abstract »|Full Text »|PDF »
Short Vegetative Phase-Like MADS-Box Genes Inhibit Floral Meristem Identity in Barley.
B. Trevaskis, M. Tadege, M. N. Hemming, W. J. Peacock, E. S. Dennis, and C. Sheldon (2007)
Plant Physiology
143, 225-235
|Abstract »|Full Text »|PDF »
From the Cover: The wheat and barley vernalization gene VRN3 is an orthologue of FT.
L. Yan, D. Fu, C. Li, A. Blechl, G. Tranquilli, M. Bonafede, A. Sanchez, M. Valarik, S. Yasuda, and J. Dubcovsky (2006)
PNAS
103, 19581-19586
|Abstract »|Full Text »|PDF »
delayed flowering1 Encodes a Basic Leucine Zipper Protein That Mediates Floral Inductive Signals at the Shoot Apex in Maize.
M. G. Muszynski, T. Dam, B. Li, D. M. Shirbroun, Z. Hou, E. Bruggemann, R. Archibald, E. V. Ananiev, and O. N. Danilevskaya (2006)
Plant Physiology
142, 1523-1536
|Abstract »|Full Text »|PDF »
Comparative Mapping of Growth Habit, Plant Height, and Flowering QTLs in Two Interspecific Families of Leymus.
S. R. Larson, X. Wu, T. A. Jones, K. B. Jensen, N. J. Chatterton, B. L. Waldron, J. G. Robins, B. S. Bushman, and A. J. Palazzo (2006)
Crop Sci.
46, 2526-2539
|Abstract »|Full Text »|PDF »
CONSTANS and the CCAAT Box Binding Complex Share a Functionally Important Domain and Interact to Regulate Flowering of Arabidopsis.
S. Wenkel, F. Turck, K. Singer, L. Gissot, J. Le Gourrierec, A. Samach, and G. Coupland (2006)
PLANT CELL
18, 2971-2984
|Abstract »|Full Text »|PDF »
RNA Interference-Based Gene Silencing as an Efficient Tool for Functional Genomics in Hexaploid Bread Wheat.
High-Resolution Radiation Hybrid Map of Wheat Chromosome 1D.
V. Kalavacharla, K. Hossain, Y. Gu, O. Riera-Lizarazu, M. I. Vales, S. Bhamidimarri, J. L. Gonzalez-Hernandez, S. S. Maan, and S. F. Kianian (2006)
Genetics
173, 1089-1099
|Abstract »|Full Text »|PDF »
The transcription factor FLC confers a flowering response to vernalization by repressing meristem competence and systemic signaling in Arabidopsis..
I. Searle, Y. He, F. Turck, C. Vincent, F. Fornara, S. Krober, R. A. Amasino, and G. Coupland (2006)
Genes & Dev.
20, 898-912
|Abstract »|Full Text »|PDF »
Characterization of the Vernalization Response in Lolium perenne by a cDNA Microarray Approach.
S. Ciannamea, J. Busscher-Lange, S. de Folter, G. C. Angenent, and R. G. H. Immink (2006)
Plant Cell Physiol.
47, 481-492
|Abstract »|Full Text »|PDF »
HvVRN2 Responds to Daylength, whereas HvVRN1 Is Regulated by Vernalization and Developmental Status.
B. Trevaskis, M. N. Hemming, W. J. Peacock, and E. S. Dennis (2006)
Plant Physiology
140, 1397-1405
|Abstract »|Full Text »|PDF »
TaVRT-2, a Member of the StMADS-11 Clade of Flowering Repressors, Is Regulated by Vernalization and Photoperiod in Wheat.
N. A. Kane, J. Danyluk, G. Tardif, F. Ouellet, J.-F. Laliberte, A. E. Limin, D. B. Fowler, and F. Sarhan (2005)
Plant Physiology
138, 2354-2363
|Abstract »|Full Text »|PDF »
Regulation of VRN-1 Vernalization Genes in Normal and Transgenic Polyploid Wheat.
A. Loukoianov, L. Yan, A. Blechl, A. Sanchez, and J. Dubcovsky (2005)
Plant Physiology
138, 2364-2373
|Abstract »|Full Text »|PDF »
DNA Rearrangement in Orthologous Orp Regions of the Maize, Rice and Sorghum Genomes.
J. Ma, P. SanMiguel, J. Lai, J. Messing, and J. L. Bennetzen (2005)
Genetics
170, 1209-1220
|Abstract »|Full Text »|PDF »
Flowering of the Grass Lolium perenne. Effects of Vernalization and Long Days on Gibberellin Biosynthesis and Signaling.
C. P. MacMillan, C. A. Blundell, and R. W. King (2005)
Plant Physiology
138, 1794-1806
|Abstract »|Full Text »|PDF »
Conservation of Arabidopsis Flowering Genes in Model Legumes.
V. Hecht, F. Foucher, C. Ferrandiz, R. Macknight, C. Navarro, J. Morin, M. E. Vardy, N. Ellis, J. P. Beltran, C. Rameau, et al. (2005)
Plant Physiology
137, 1420-1434
|Abstract »|Full Text »|PDF »
Abiotic Stress Tolerance in Grasses. From Model Plants to Crop Plants.
M. Tester and A. Bacic (2005)
Plant Physiology
137, 791-793
|Full Text »|PDF »
Low levels of linkage disequilibrium in wild barley (Hordeum vulgare ssp. spontaneum) despite high rates of self-fertilization.
P. L. Morrell, D. M. Toleno, K. E. Lundy, and M. T. Clegg (2005)
PNAS
102, 2442-2447
|Abstract »|Full Text »|PDF »
Fine Mapping of a Grain-Weight Quantitative Trait Locus in the Pericentromeric Region of Rice Chromosome 3.
A 2500-Locus Bin Map of Wheat Homoeologous Group 5 Provides Insights on Gene Distribution and Colinearity With Rice.
A. M. Linkiewicz, L. L. Qi, B. S. Gill, A. Ratnasiri, B. Echalier, S. Chao, G. R. Lazo, D. D. Hummel, O. D. Anderson, E. D. Akhunov, et al. (2004)
Genetics
168, 665-676
|Abstract »|Full Text »|PDF »
A Workshop Report on Wheat Genome Sequencing: International Genome Research on Wheat Consortium.
B. S. Gill, R. Appels, A.-M. Botha-Oberholster, C. R. Buell, J. L. Bennetzen, B. Chalhoub, F. Chumley, J. Dvorak, M. Iwanaga, B. Keller, et al. (2004)
Genetics
168, 1087-1096
|Abstract »|Full Text »|PDF »
Vernalization, Competence, and the Epigenetic Memory of Winter.