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Science 27 February 2004:
Vol. 303. no. 5662, pp. 1358 - 1361
DOI: 10.1126/science.1094226
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Reports

Legionella Effectors That Promote Nonlytic Release from Protozoa

John Chen,1 Karim Suwwan de Felipe,2 Margaret Clarke,3 Hao Lu,3 O. Roger Anderson,4 Gil Segal,5 Howard A. Shuman1*

Legionella pneumophila, the bacterial agent of legionnaires' disease, replicates intracellularly within a specialized vacuole of mammalian and protozoan host cells. Little is known about the specialized vacuole except that the Icm/Dot type IVsecretion system is essential for its formation and maintenance. The Legionella genome database contains two open reading frames encoding polypeptides (LepA and LepB) with predicted coiled-coil regions and weak homology to SNAREs; these are delivered to host cells by an Icm/Dot–dependent mechanism. Analysis of mutant strains suggests that the Lep proteins may enable the Legionella to commandeer a protozoan exocytic pathway for dissemination of the pathogen.

1 Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA.
2 Integrated Program in Cellular, Molecular and Biophysical Studies, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA.
3 Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, 825 N.E. 13th Street, Oklahoma City, OK 73104–5046, USA.
4 Department of Biology, Lamont-Doherty Earth Observatory, Columbia University, 61 Route 9W, Palisades, NY 10964–1000, USA.
5 Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.

* To whom correspondence should be addressed. E-mail: has7{at}columbia.edu

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