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Originally published in Science Express on 8 January 2004
Science 13 February 2004:
Vol. 303. no. 5660, pp. 1020 - 1023
DOI: 10.1126/science.1091611
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Reports

Instructive Role of Wnt/ß-Catenin in Sensory Fate Specification in Neural Crest Stem Cells

Hye-Youn Lee,1* Maurice Kléber,1* Lisette Hari,1 Véronique Brault,2{dagger} Ueli Suter,1 Makoto M. Taketo,3 Rolf Kemler,2 Lukas Sommer1{ddagger}

Wnt signaling has recently emerged as a key factor in controlling stem cell expansion. In contrast, we show here that Wnt/ß-catenin signal activation in emigrating neural crest stem cells (NCSCs) has little effect on the population size and instead regulates fate decisions. Sustained ß-catenin activity in neural crest cells promotes the formation of sensory neural cells in vivo at the expense of virtually all other neural crest derivatives. Moreover, Wnt1 is able to instruct early NCSCs (eNCSCs) to adopt a sensory neuronal fate in a ß-catenin–dependent manner. Thus, the role of Wnt/ß-catenin in stem cells is cell-type dependent.

1 Institute of Cell Biology, Department of Biology, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zurich, Switzerland.
2 Department of Molecular Embryology, Max-Planck Institute of Immunobiology, Stuebeweg 51, D-79108 Freiburg, Germany.
3 Department of Pharmacology, Graduate School of Medicine, Kyoto University, Yoshida-Konoé-cho, Sakyo, Kyoto 606-8501, Japan.


* These authors contributed equally to this work.

{dagger} Present address: CNRS.GEM, FRE 2358, Genetique Experimentale et Moleculaire, Institut de Transgenose, 3B rue de la Ferollerie, F-45071 Orleans Cedex 2, France.

{ddagger} To whom correspondence should be addressed. E-mail: lukas.sommer{at}cell.biol.ethz.ch

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