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Science 30 January 2004:
Vol. 303. no. 5658, pp. 679 - 681
DOI: 10.1126/science.1092600
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Reports

Lacticin 481: In Vitro Reconstitution of Lantibiotic Synthetase Activity

Lili Xie, Leah M. Miller, Champak Chatterjee, Olga Averin, Neil L. Kelleher,* Wilfred A. van der Donk*

The lantibiotic lacticin 481 is synthesized on ribosomes as a prepeptide (LctA) and posttranslationally modified to its mature form. These modifications include dehydration of serines and threonines, followed by intramolecular addition of cysteines to the unsaturated amino acids, which generates cyclic thioethers. This process breaks eight chemical bonds and forms six newbonds and is catalyzed by one enzyme, LctM. We have characterized the in vitro activity of LctM, which completely processed a series of LctA mutants, displaying a permissive substrate specificity that holds promise for antibiotic engineering.

Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL61801, USA.

* To whom correspondence should be addressed. E-mail: vddonk{at}uiuc.edu

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