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Science 10 October 2003:
Vol. 302. no. 5643, pp. 286 - 290
DOI: 10.1126/science.1086997
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Reports

A Functional Link Between RuBisCO-like Protein of Bacillus and Photosynthetic RuBisCO

Hiroki Ashida,1 Yohtaro Saito,1 Chojiro Kojima,1 Kazuo Kobayashi,1 Naotake Ogasawara,1 Akiho Yokota1,2*

The genomes of several nonphotosynthetic bacteria, such as Bacillus subtilis, and some Archaea include genes for proteins with sequence homology to the large subunit of ribulose bisphosphate carboxylase/oxygenase (RuBisCO). We found that such a RuBisCO-like protein (RLP) from B. subtilis catalyzed the 2,3-diketo-5-methylthiopentyl-1-phosphate enolase reaction in the methionine salvage pathway. A growth-defective mutant, in which the gene for this RLP had been disrupted, was rescued by the gene for RuBisCOfrom the photosynthetic bacterium Rhodospirillum rubrum. Thus, the photosynthetic RuBisCO from R. rubrum retains the ability to function in the methionine salvage pathway in B. subtilis.

1 Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Ikoma, Nara 630-0101, Japan.
2 Plant Molecular Physiology Laboratory, Research Institute for Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Kizu, Sorakugun, Kyoto 619-0292, Japan.

* To whom correspondence should be addressed. E-mail: yokota{at}bs.aist-nara.ac.jp

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Science. ISSN 0036-8075 (print), 1095-9203 (online)