Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 3 October 2003:
Vol. 302. no. 5642, pp. 106 - 109
DOI: 10.1126/science.1088074
Prev | Table of Contents | Next

Reports

Self-Assembly of Proteins into Designed Networks

Philippe Ringler and Georg E. Schulz*

A C4-symmetric tetrameric aldolase was used to produce a quadratic network consisting of the enzyme as a rigid four-way connector and stiff streptavidin rods as spacers. Each aldolase subunit was furnished with a His6 tag for oriented binding to a planar surface and two tethered biotins for binding streptavidin in an oriented manner. The networks were improved by starting with composite units and also by binding to nickel–nitrilotriacetic acid–lipid monolayers. The mesh was adjustable in 5-nanometer increments. The production of a net with switchable mesh was initiated with the use of a calcium ion–containing ß-helix spacer that denatured on calcium ion depletion.

Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, D-79104 Freiburg im Breisgau, Germany.

* To whom correspondence should be addressed. E-mail: schulz{at}bio.chemie.uni-freiburg.de

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Block Copolymer Assembly via Kinetic Control.
H. Cui, Z. Chen, S. Zhong, K. L. Wooley, and D. J. Pochan (2007)
Science 317, 647-650
   Abstract »    Full Text »    PDF »
Design and application of stimulus-responsive peptide systems.
K. Chockalingam, M. Blenner, and S. Banta (2007)
Protein Eng. Des. Sel. 20, 155-161
   Abstract »    Full Text »    PDF »
Building Programmable Jigsaw Puzzles with RNA.
A. Chworos, I. Severcan, A. Y. Koyfman, P. Weinkam, E. Oroudjev, H. G. Hansma, and L. Jaeger (2004)
Science 306, 2068-2072
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)