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Science 3 October 2003:
Vol. 302. no. 5642, pp. 103 - 106
DOI: 10.1126/science.1086652
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Reports

Talin Binding to Integrin ß Tails: A Final Common Step in Integrin Activation

Seiji Tadokoro,1 Sanford J. Shattil,1 Koji Eto,1 Vera Tai,1 Robert C. Liddington,2 José M. de Pereda,2 Mark H. Ginsberg,1* David A. Calderwood1

Control of integrin affinity for ligands (integrin activation) is essential for normal cell adhesion, migration, and assembly of an extracellular matrix. Integrin activation is usually mediated through the integrin ß subunit cytoplasmic tail and can be regulated by many different biochemical signaling pathways. We report that specific binding of the cytoskeletal protein talin to integrin ß subunit cytoplasmic tails leads to the conformational rearrangements of integrin extracellular domains that increase their affinity. Thus, regulated binding of talin to integrin ß tails is a final common element of cellular signaling cascades that control integrin activation.

1 Department of Cell Biology, The Scripps Research Institute, The Burnham Institute, La Jolla, CA 92037, USA.
2 Program in Cell Adhesion, The Burnham Institute, La Jolla, CA 92037, USA.

* To whom correspondence should be addressed. E-mail: ginsberg{at}scripps.edu

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Phospholipase C{epsilon} Suppresses Integrin Activation.
Y. Lad, B. McHugh, P. S. Hodkinson, A. C. MacKinnon, C. Haslett, M. H. Ginsberg, and T. Sethi (2006)
J. Biol. Chem. 281, 29501-29512
   Abstract »    Full Text »    PDF »
Tyrosine Phosphorylation of the Integrin beta3 Subunit Regulates beta3 Cleavage by Calpain.
X. Xi, P. Flevaris, A. Stojanovic, A. Chishti, D. R. Phillips, S. C. T. Lam, and X. Du (2006)
J. Biol. Chem. 281, 29426-29430
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Initiation of Attachment and Generation of Mature Focal Adhesions by Integrin-containing Filopodia in Cell Spreading.
M. A. Partridge and E. E. Marcantonio (2006)
Mol. Biol. Cell 17, 4237-4248
   Abstract »    Full Text »    PDF »
Genetic analysis of {beta}1 integrin "activation motifs" in mice.
A. Czuchra, H. Meyer, K. R. Legate, C. Brakebusch, and R. Fassler (2006)
J. Cell Biol. 174, 889-899
   Abstract »    Full Text »    PDF »
Perturbing integrin function inhibits microtubule growth from centrosomes, spindle assembly, and cytokinesis.
C. G. Reverte, A. Benware, C. W. Jones, and S. E. LaFlamme (2006)
J. Cell Biol. 174, 491-497
   Abstract »    Full Text »    PDF »


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