Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
A Seven-Transmembrane RGS Protein That Modulates Plant Cell Proliferation
Jin-Gui Chen,1Francis S. Willard,2Jirong Huang,1Jiansheng Liang,1*Scott A. Chasse,3Alan M. Jones,1David P. Siderovski2
G protein–coupled receptors (GPCRs) at the cell surfaceactivate heterotrimeric G proteins by inducing the G proteinalpha (G) subunit to exchange guanosine diphosphate for guanosinetriphosphate. Regulators of G protein signaling (RGS) proteinsaccelerate the deactivation of G subunits to reduce GPCR signaling.Here we identified an RGS protein (AtRGS1) in Arabidopsis thathas a predicted structure similar to a GPCR as well as an RGSbox with GTPase accelerating activity. Expression of AtRGS1complemented the pheromone supersensitivity phenotype of a yeastRGS mutant, sst2. Loss of AtRGS1 increased the activity of theArabidopsis G subunit, resulting in increased cell elongationin hypocotyls in darkness and increased cell production in rootsgrown in light. These findings suggest that AtRGS1 is a criticalmodulator of plant cell proliferation.
1 Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599–3280, USA. 2 Department of Pharmacology, Lineberger Comprehensive Cancer Center, and UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599–7365, USA. 3 Department of Biochemistry and Biophysics, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599–7260, USA.
* Permanent address: College of Bioscience and Biotechnology,Yangzhou University, Yangzhou 225009, People's Republic of China.
To whom correspondence should be addressed. E-mail: alan_jones{at}unc.edu
Loss-of-Function Mutations in the Arabidopsis Heterotrimeric G-protein {alpha} Subunit Enhance the Developmental Defects of Brassinosteroid Signaling and Biosynthesis Mutants.
Y. Gao, S. Wang, T. Asami, and J.-G. Chen (2008)
Plant Cell Physiol.
49, 1013-1024
|Abstract »|Full Text »|PDF »
Abscisic acid regulation of guard-cell K+ and anion channels in G{beta}- and RGS-deficient Arabidopsis lines.
L.-M. Fan, W. Zhang, J.-G. Chen, J. P. Taylor, A. M. Jones, and S. M. Assmann (2008)
PNAS
105, 8476-8481
|Abstract »|Full Text »|PDF »
Characterization of the Arabidopsis Heterotrimeric G Protein.
S. Wang, S. M. Assmann, and N. V. Fedoroff (2008)
J. Biol. Chem.
283, 13913-13922
|Abstract »|Full Text »|PDF »
GTPase acceleration as the rate-limiting step in Arabidopsis G protein-coupled sugar signaling.
C. A. Johnston, J. P. Taylor, Y. Gao, A. J. Kimple, J. C. Grigston, J.-G. Chen, D. P. Siderovski, A. M. Jones, and F. S. Willard (2007)
PNAS
104, 17317-17322
|Abstract »|Full Text »|PDF »
Analysis of Leaf Development in fugu Mutants of Arabidopsis Reveals Three Compensation Modes That Modulate Cell Expansion in Determinate Organs.
A. Ferjani, G. Horiguchi, S. Yano, and H. Tsukaya (2007)
Plant Physiology
144, 988-999
|Abstract »|Full Text »|PDF »
A Golgi-localized Hexose Transporter Is Involved in Heterotrimeric G Protein-mediated Early Development in Arabidopsis.
H. X. Wang, R. R. Weerasinghe, T. D. Perdue, N. G. Cakmakci, J. P. Taylor, W. F. Marzluff, and A. M. Jones (2006)
Mol. Biol. Cell
17, 4257-4269
|Abstract »|Full Text »|PDF »
RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis.
J.-G. Chen, H. Ullah, B. Temple, J. Liang, J. Guo, J. M. Alonso, J. R. Ecker, and A. M. Jones (2006)
J. Exp. Bot.
57, 2697-2708
|Abstract »|Full Text »|PDF »
Differential Roles of Arabidopsis Heterotrimeric G-Protein Subunits in Modulating Cell Division in Roots.
Overexpression of the regulator of G-protein signalling protein enhances ABA-mediated inhibition of root elongation and drought tolerance in Arabidopsis.
The Plastid Protein THYLAKOID FORMATION1 and the Plasma Membrane G-Protein GPA1 Interact in a Novel Sugar-Signaling Mechanism in Arabidopsis.
J. Huang, J. P. Taylor, J.-G. Chen, J. F. Uhrig, D. J. Schnell, T. Nakagawa, K. L. Korth, and A. M. Jones (2006)
PLANT CELL
18, 1226-1238
|Abstract »|Full Text »|PDF »
G-Protein Complex Mutants Are Hypersensitive to Abscisic Acid Regulation of Germination and Postgermination Development.
S. Pandey, J.-G. Chen, A. M. Jones, and S. M. Assmann (2006)
Plant Physiology
141, 243-256
|Abstract »|Full Text »|PDF »
The Regulator of G-Protein Signaling Proteins Involved in Sugar and Abscisic Acid Signaling in Arabidopsis Seed Germination.
Y. Chen, F. Ji, H. Xie, J. Liang, and J. Zhang (2006)
Plant Physiology
140, 302-310
|Abstract »|Full Text »|PDF »
A novel gene family in Arabidopsis encoding putative heptahelical transmembrane proteins homologous to human adiponectin receptors and progestin receptors.
GCR1 Can Act Independently of Heterotrimeric G-Protein in Response to Brassinosteroids and Gibberellins in Arabidopsis Seed Germination.
J.-G. Chen, S. Pandey, J. Huang, J. M. Alonso, J. R. Ecker, S. M. Assmann, and A. M. Jones (2004)
Plant Physiology
135, 907-915
|Abstract »|Full Text »|PDF »
Arabidopsis Phospholipase D{alpha}1 Interacts with the Heterotrimeric G-protein {alpha}-Subunit through a Motif Analogous to the DRY Motif in G-protein-coupled Receptors.
) subunit to exchange guanosine diphosphate for guanosine triphosphate. Regulators of G protein signaling (RGS) proteins accelerate the deactivation of G
. Loss of AtRGS1 increased the activity of the Arabidopsis G
To whom correspondence should be addressed. E-mail: 
