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Science 22 August 2003:
Vol. 301. no. 5636, pp. 1094 - 1096
DOI: 10.1126/science.1085712
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Reports

Tracking FACT and the RNA Polymerase II Elongation Complex Through Chromatin in Vivo

Abbie Saunders,1 Janis Werner,1 Erik D. Andrulis,1* Takahiro Nakayama,2 Susumu Hirose,2 Danny Reinberg,3 John T. Lis1{dagger}

RNA polymerase II (Pol II) transcription through nucleosomes is facilitated in vitro by the protein complex FACT (Facilitates Chromatin Transcription). Here we show that FACT is associated with actively transcribed Pol II genes on Drosophila polytene chromosomes. FACT displays kinetics of recruitment and of chromosome tracking in vivo similar to Pol II and elongation factors Spt5 and Spt6. Interestingly, FACT does not colocalize with Pol III–transcribed genes, which are known to undergo nucleosome transfer rather than disassembly in vitro. Our observations are consistent with FACT being restricted to transcription that involves nucleosome disassembly mechanisms.

1 Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14850, USA.
2 Department of Developmental Genetics, National Institute of Genetics, 1111 Yata, Mishima, Shizuokaken 411-8540, Japan.
3 Howard Hughes Medical Institute, Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA.



* Present address: Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106–4960, USA.

{dagger} To whom correspondence should be addressed. E-mail: jtl10{at}cornell.edu

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