Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Induction of Tumors in Mice by Genomic Hypomethylation
François Gaudet,1,2,3J. Graeme Hodgson,4Amir Eden,1Laurie Jackson-Grusby,1Jessica Dausman,1Joe W. Gray,4Heinrich Leonhardt,2,3Rudolf Jaenisch1*
Genome-wide DNA hypomethylation occurs in many human cancers,but whether this epigenetic change is a cause or consequenceof tumorigenesis has been unclear. To explore this phenomenon,we generated mice carrying a hypomorphic DNA methyltransferase1 (Dnmt1) allele, which reduces Dnmt1 expression to 10% of wild-typelevels and results in substantial genome-wide hypomethylationin all tissues. The mutant mice were runted at birth, and at4 to 8 months of age they developed aggressive T cell lymphomasthat displayed a high frequency of chromosome 15 trisomy. Theseresults indicate that DNA hypomethylation plays a causal rolein tumor formation, possibly by promoting chromosomal instability.
1 Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA. 2 Ludwig Maximilians University, Department of Biology II, 80336 Munich, Germany. 3 Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany. 4 Department of Laboratory Medicine and UCSF Comprehensive Cancer Center, University of California, 2340 Sutter Street, San Francisco, CA 94143, USA.
* To whom correspondence should be addressed. E-mail: jaenisch{at}wi.mit.edu
The editors suggest the following Related Resources on Science sites:
In Science Magazine
TECHNICAL COMMENTS
Allen S. Yang, Marcos R.H. Estecio, Guillermo Garcia-Manero, Hagop M. Kantarjian, and Jean-Pierre J. Issa (14 November 2003) Science302 (5648), 1153b.
[DOI: 10.1126/science.1089523] |Full Text »|PDF »
TECHNICAL COMMENTS
Amir Eden, François Gaudet, and Rudolf Jaenisch (14 November 2003) Science302 (5648), 1153c.
[DOI: 10.1126/science.1089838] |Full Text »|PDF »
PERSPECTIVES
Christoph Lengauer (18 April 2003) Science300 (5618), 442.
[DOI: 10.1126/science.1084468] |Summary »|Full Text »|PDF »
BREVIA
Amir Eden, François Gaudet, Alpana Waghmare, and Rudolf Jaenisch (18 April 2003) Science300 (5618), 455.
[DOI: 10.1126/science.1083557] |Full Text »|PDF »|Supporting Online Material »
W. G. Nelson, A. M. De Marzo, and S. Yegnasubramanian (2009)
Endocrinology
150, 3991-4002
|Abstract »|Full Text »|PDF »
Epigenetic changes during disease progression in a murine model of human chronic lymphocytic leukemia.
S.-S. Chen, A. Raval, A. J. Johnson, E. Hertlein, T.-H. Liu, V. X. Jin, M. H. Sherman, S.-J. Liu, D. W. Dawson, K. E. Williams, et al. (2009)
PNAS
106, 13433-13438
|Abstract »|Full Text »|PDF »
PCR-Based Methods for Detecting Single-Locus DNA Methylation Biomarkers in Cancer Diagnostics, Prognostics, and Response to Treatment.
Folate Supplementation Limits the Aggressiveness of Glioma via the Remethylation of DNA Repeats Element and Genes Governing Apoptosis and Proliferation.
E. Hervouet, E. Debien, L. Campion, J. Charbord, J. Menanteau, F. M. Vallette, and P.-F. Cartron (2009)
Clin. Cancer Res.
15, 3519-3529
|Abstract »|Full Text »|PDF »
Epigenetics: Connecting Environment and Genotype to Phenotype and Disease.
S.P. Barros and S. Offenbacher (2009)
Journal of Dental Research
88, 400-408
|Abstract »|Full Text »|PDF »
Global DNA Hypomethylation (LINE-1) in the Normal Colon and Lifestyle Characteristics and Dietary and Genetic Factors.
J. C. Figueiredo, M. V. Grau, K. Wallace, A. J. Levine, L. Shen, R. Hamdan, X. Chen, R. S. Bresalier, G. McKeown-Eyssen, R. W. Haile, et al. (2009)
Cancer Epidemiol. Biomarkers Prev.
18, 1041-1049
|Abstract »|Full Text »|PDF »
Epigenetics: poly(ADP-ribosyl)ation of PARP-1 regulates genomic methylation patterns.
Epigenetic profiling reveals etiologically distinct patterns of DNA methylation in head and neck squamous cell carcinoma.
C. J. Marsit, B. C. Christensen, E. A. Houseman, M. R. Karagas, M. R. Wrensch, R.-F. Yeh, H. H. Nelson, J. L. Wiemels, S. Zheng, M. R. Posner, et al. (2009)
Carcinogenesis
30, 416-422
|Abstract »|Full Text »|PDF »
Association between frequent CpG island methylation and HER2 amplification in human breast cancers.
K. Terada, E. Okochi-Takada, S. Akashi-Tanaka, K. Miyamoto, K. Taniyama, H. Tsuda, K. Asada, M. Kaminishi, and T. Ushijima (2009)
Carcinogenesis
30, 466-471
|Abstract »|Full Text »|PDF »
A Cohort Study of Tumoral LINE-1 Hypomethylation and Prognosis in Colon Cancer.
S. Ogino, K. Nosho, G. J. Kirkner, T. Kawasaki, A. T. Chan, E. S. Schernhammer, E. L. Giovannucci, and C. S. Fuchs (2008)
J Natl Cancer Inst
100, 1734-1738
|Abstract »|Full Text »|PDF »
Chromosome 12p Deletions in TEL-AML1 Childhood Acute Lymphoblastic Leukemia Are Associated with Retrotransposon Elements and Occur Postnatally.
J. L. Wiemels, J. Hofmann, M. Kang, R. Selzer, R. Green, M. Zhou, S. Zhong, L. Zhang, M. T. Smith, C. Marsit, et al. (2008)
Cancer Res.
68, 9935-9944
|Abstract »|Full Text »|PDF »
DNA Hypomethylation Arises Later in Prostate Cancer Progression than CpG Island Hypermethylation and Contributes to Metastatic Tumor Heterogeneity.
S. Yegnasubramanian, M. C. Haffner, Y. Zhang, B. Gurel, T. C. Cornish, Z. Wu, R. A. Irizarry, J. Morgan, J. Hicks, T. L. DeWeese, et al. (2008)
Cancer Res.
68, 8954-8967
|Abstract »|Full Text »|PDF »
LINE-1 Hypomethylation Is Associated with Increased CpG Island Methylation in Helicobacter pylori-Related Enlarged-Fold Gastritis.
E. Yamamoto, M. Toyota, H. Suzuki, Y. Kondo, T. Sanomura, Y. Murayama, M. Ohe-Toyota, R. Maruyama, M. Nojima, M. Ashida, et al. (2008)
Cancer Epidemiol. Biomarkers Prev.
17, 2555-2564
|Abstract »|Full Text »|PDF »
Genome-Wide Analysis in a Murine Dnmt1 Knockdown Model Identifies Epigenetically Silenced Genes in Primary Human Pituitary Tumors.
K. J. Dudley, K. Revill, P. Whitby, R. N. Clayton, and W. E. Farrell (2008)
Mol. Cancer Res.
6, 1567-1574
|Abstract »|Full Text »|PDF »
Kaiso Contributes to DNA Methylation-Dependent Silencing of Tumor Suppressor Genes in Colon Cancer Cell Lines.
E. C. Lopes, E. Valls, M. E. Figueroa, A. Mazur, F.-G. Meng, G. Chiosis, P. W. Laird, N. Schreiber-Agus, J. M. Greally, E. Prokhortchouk, et al. (2008)
Cancer Res.
68, 7258-7263
|Abstract »|Full Text »|PDF »
Long-term Epigenetic Therapy with Oral Zebularine Has Minimal Side Effects and Prevents Intestinal Tumors in Mice.
C. B. Yoo, J. C. Chuang, H.-M. Byun, G. Egger, A. S. Yang, L. Dubeau, T. Long, P. W. Laird, V. E. Marquez, and P. A. Jones (2008)
Cancer Prevention Research
1, 233-240
|Abstract »|Full Text »|PDF »
CCCTC-binding Factor Activates PARP-1 Affecting DNA Methylation Machinery.
T. Guastafierro, B. Cecchinelli, M. Zampieri, A. Reale, G. Riggio, O. Sthandier, G. Zupi, L. Calabrese, and P. Caiafa (2008)
J. Biol. Chem.
283, 21873-21880
|Abstract »|Full Text »|PDF »
Expression of t-DARPP Mediates Trastuzumab Resistance in Breast Cancer Cells.
A. Belkhiri, A. A. Dar, D. F. Peng, M. H. Razvi, C. Rinehart, C. L. Arteaga, and W. El-Rifai (2008)
Clin. Cancer Res.
14, 4564-4571
|Abstract »|Full Text »|PDF »
Intra-individual Change Over Time in DNA Methylation With Familial Clustering.
H. T. Bjornsson, M. I. Sigurdsson, M. D. Fallin, R. A. Irizarry, T. Aspelund, H. Cui, W. Yu, M. A. Rongione, T. J. Ekstrom, T. B. Harris, et al. (2008)
JAMA
299, 2877-2883
|Abstract »|Full Text »|PDF »
15-Lipoxygenase-1 transcriptional silencing by DNA methyltransferase-1 independently of DNA methylation.
X. Zuo, L. Shen, J.-P. Issa, O. Moy, J. S. Morris, S. M. Lippman, and I. Shureiqi (2008)
FASEB J
22, 1981-1992
|Abstract »|Full Text »|PDF »
Epigenetic Modification of CCAAT/Enhancer Binding Protein {alpha} Expression in Acute Myeloid Leukemia.
B. Hackanson, K. L. Bennett, R. M. Brena, J. Jiang, R. Claus, S.-S. Chen, N. Blagitko-Dorfs, K. Maharry, S. P. Whitman, T. D. Schmittgen, et al. (2008)
Cancer Res.
68, 3142-3151
|Abstract »|Full Text »|PDF »
DNA Methylation as a Therapeutic Target in Cancer.
Line Region Hypomethylation Is Associated with Lifestyle and Differs by Human Papillomavirus Status in Head and Neck Squamous Cell Carcinomas.
C. S. Furniss, C. J. Marsit, E. A. Houseman, K. Eddy, and K. T. Kelsey (2008)
Cancer Epidemiol. Biomarkers Prev.
17, 966-971
|Abstract »|Full Text »|PDF »
Silent Assassin: Oncogenic Ras Directs Epigenetic Inactivation of Target Genes.
M. Esteller (2008)
N. Engl. J. Med.
358, 1148-1159
|Full Text »|PDF »
High-resolution mapping of DNA hypermethylation and hypomethylation in lung cancer.
T. A. Rauch, X. Zhong, X. Wu, M. Wang, K. H. Kernstine, Z. Wang, A. D. Riggs, and G. P. Pfeifer (2008)
PNAS
105, 252-257
|Abstract »|Full Text »|PDF »
Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing.
H. G. Linhart, H. Lin, Y. Yamada, E. Moran, E. J. Steine, S. Gokhale, G. Lo, E. Cantu, M. Ehrich, T. He, et al. (2007)
Genes & Dev.
21, 3110-3122
|Abstract »|Full Text »|PDF »
Annurca Apple Polyphenols Have Potent Demethylating Activity and Can Reactivate Silenced Tumor Suppressor Genes in Colorectal Cancer Cells.
L. Fini, M. Selgrad, V. Fogliano, G. Graziani, M. Romano, E. Hotchkiss, Y. A. Daoud, E. B. De Vol, C. R. Boland, and L. Ricciardiello (2007)
J. Nutr.
137, 2622-2628
|Abstract »|Full Text »|PDF »
Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation.
L. Schermelleh, A. Haemmer, F. Spada, N. Rosing, D. Meilinger, U. Rothbauer, M. C. Cardoso, and H. Leonhardt (2007)
Nucleic Acids Res.
35, 4301-4312
|Abstract »|Full Text »|PDF »
Biological Functions of DNA Methyltransferase 1 Require Its Methyltransferase Activity.
Orphan Nuclear Receptor Constitutive Active/Androstane Receptor-Mediated Alterations in DNA Methylation during Phenobarbital Promotion of Liver Tumorigenesis.
J. M. Phillips, Y. Yamamoto, M. Negishi, R. R. Maronpot, and J. I. Goodman (2007)
Toxicol. Sci.
96, 72-82
|Abstract »|Full Text »|PDF »
Differences between human embryonic stem cell lines.
DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells.
F. Spada, A. Haemmer, D. Kuch, U. Rothbauer, L. Schermelleh, E. Kremmer, T. Carell, G. Langst, and H. Leonhardt (2007)
J. Cell Biol.
176, 565-571
|Abstract »|Full Text »|PDF »
Epigenetic regulation of human cancer/testis antigen gene, HAGE, in chronic myeloid leukemia.
J. Roman-Gomez, A. Jimenez-Velasco, X. Agirre, J. A. Castillejo, G. Navarro, E. San Jose-Eneriz, L. Garate, L. Cordeu, F. Cervantes, F. Prosper, et al. (2007)
Haematologica
92, 153-162
|Abstract »|Full Text »|PDF »
Global DNA Methylation Level in Whole Blood as a Biomarker in Head and Neck Squamous Cell Carcinoma.
D. Ting Hsiung, C. J. Marsit, E. A. Houseman, K. Eddy, C. S. Furniss, M. D. McClean, and K. T. Kelsey (2007)
Cancer Epidemiol. Biomarkers Prev.
16, 108-114
|Abstract »|Full Text »|PDF »
Prevention of mouse lung tumors and modulation of DNA methylation by combined treatment with budesonide and R115777 (ZarnestraMT).
F. S. Alyaqoub, L. Tao, P. M. Kramer, V. E. Steele, R. A. Lubet, W. T. Gunning, and M. A. Pereira (2007)
Carcinogenesis
28, 124-129
|Abstract »|Full Text »|PDF »
DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains.
K. Monier, S. Mouradian, and K. F. Sullivan (2007)
J. Cell Sci.
120, 101-114
|Abstract »|Full Text »|PDF »
Modifying the Epigenome as a Therapeutic Strategy in Myelodysplasia.
Epigenetic Silencing of Novel Tumor Suppressors in Malignant Melanoma.
V. Muthusamy, S. Duraisamy, C. M. Bradbury, C. Hobbs, D. P. Curley, B. Nelson, and M. Bosenberg (2006)
Cancer Res.
66, 11187-11193
|Abstract »|Full Text »|PDF »
Prevention of mouse lung tumors and modulation of DNA methylation by combined treatment with budesonide and R115777 (ZarnestraMT).
F. S. Alyaqoub, L. Tao, P. M. Kramer, V. E. Steele, R. A. Lubet, W. T. Gunning, and M. A. Pereira (2006)
Carcinogenesis
27, 2442-2447
|Abstract »|Full Text »|PDF »
Dysregulated TCL1 requires the germinal center and genome instability for mature B-cell transformation.
R. R. Shen, D. O. Ferguson, M. Renard, K. K. Hoyer, U. Kim, X. Hao, F. W. Alt, R. G. Roeder, H. C. Morse III, and M. A. Teitell (2006)
Blood
108, 1991-1998
|Abstract »|Full Text »|PDF »
Testing gene function early in the B cell lineage in mb1-cre mice.
E. Hobeika, S. Thiemann, B. Storch, H. Jumaa, P. J. Nielsen, R. Pelanda, and M. Reth (2006)
PNAS
103, 13789-13794
|Abstract »|Full Text »|PDF »
Chromosomal Instability Correlates with Genome-wide DNA Demethylation in Human Primary Colorectal Cancers..
J. Rodriguez, J. Frigola, E. Vendrell, R.-A. Risques, M. F. Fraga, C. Morales, V. Moreno, M. Esteller, G. Capella, M. Ribas, et al. (2006)
Cancer Res.
66, 8462-9468
|Abstract »|Full Text »|PDF »
Genome-wide Hypomethylation in Human Glioblastomas Associated with Specific Copy Number Alteration, Methylenetetrahydrofolate Reductase Allele Status, and Increased Proliferation..
B. Cadieux, T.-T. Ching, S. R. VandenBerg, and J. F. Costello (2006)
Cancer Res.
66, 8469-8476
|Abstract »|Full Text »|PDF »
Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis.
V. P. Tryndyak, L. Muskhelishvili, O. Kovalchuk, R. Rodriguez-Juarez, B. Montgomery, M. I. Churchwell, S. A. Ross, F. A. Beland, and I. P. Pogribny (2006)
Carcinogenesis
27, 1713-1720
|Abstract »|Full Text »|PDF »
MAGE-A tumor antigens target p53 transactivation function through histone deacetylase recruitment and confer resistance to chemotherapeutic agents.
M. Monte, M. Simonatto, L. Y. Peche, D. R. Bublik, S. Gobessi, M. A. Pierotti, M. Rodolfo, and C. Schneider (2006)
PNAS
103, 11160-11165
|Abstract »|Full Text »|PDF »
Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b..
A. Tsumura, T. Hayakawa, Y. Kumaki, S.-i. Takebayashi, M. Sakaue, C. Matsuoka, K. Shimotohno, F. Ishikawa, E. Li, H. R. Ueda, et al. (2006)
Genes Cells
11, 805-814
|Abstract »|Full Text »|PDF »
Phenobarbital Induces Progressive Patterns of GC-Rich and Gene-Specific Altered DNA Methylation in the Liver of Tumor-Prone B6C3F1 Mice.
A. N. Bachman, J. M. Phillips, and J. I. Goodman (2006)
Toxicol. Sci.
91, 393-405
|Abstract »|Full Text »|PDF »
Altered Methylation in Gene-Specific and GC-Rich Regions of DNA Is Progressive and Nonrandom during Promotion of Skin Tumorigenesis.
A. N. Bachman, G. M. Curtin, D. J. Doolittle, and J. I. Goodman (2006)
Toxicol. Sci.
91, 406-418
|Abstract »|Full Text »|PDF »
Histone H3 lysine 9 and H4 lysine 20 trimethylation and the expression of Suv4-20h2 and Suv-39h1 histone methyltransferases in hepatocarcinogenesis induced by methyl deficiency in rats.
I. P. Pogribny, S. A. Ross, V. P. Tryndyak, M. Pogribna, L. A. Poirier, and T. V. Karpinets (2006)
Carcinogenesis
27, 1180-1186
|Abstract »|Full Text »|PDF »
DNA Methylation Changes after 5-Aza-2'-Deoxycytidine Therapy in Patients with Leukemia..
A. S. Yang, K. D. Doshi, S.-W. Choi, J. B. Mason, R. K. Mannari, V. Gharybian, R. Luna, A. Rashid, L. Shen, M. R.H. Estecio, et al. (2006)
Cancer Res.
66, 5495-5503
|Abstract »|Full Text »|PDF »
Suppression of Intestinal Neoplasia by Deletion of Dnmt3b.
H. Lin, Y. Yamada, S. Nguyen, H. Linhart, L. Jackson-Grusby, A. Meissner, K. Meletis, G. Lo, and R. Jaenisch (2006)
Mol. Cell. Biol.
26, 2976-2983
|Abstract »|Full Text »|PDF »
Transient Down-regulation of DNMT1 Methyltransferase Leads to Activation and Stable Hypomethylation of MAGE-A1 in Melanoma Cells.
A. Loriot, E. De Plaen, T. Boon, and C. De Smet (2006)
J. Biol. Chem.
281, 10118-10126
|Abstract »|Full Text »|PDF »
Epigenetic Instability and Chromosomal Instability in Hepatocellular Carcinoma.
H. Katoh, T. Shibata, A. Kokubu, H. Ojima, M. Fukayama, Y. Kanai, and S. Hirohashi (2006)
Am. J. Pathol.
168, 1375-1384
|Abstract »|Full Text »|PDF »
Roles for Dnmt3b in mammalian development: a mouse model for the ICF syndrome..
Y. Ueda, M. Okano, C. Williams, T. Chen, K. Georgopoulos, and E. Li (2006)
Development
133, 1183-1192
|Abstract »|Full Text »|PDF »
Aging in heterozygous dnmt1-deficient mice: effects on survival, the DNA methylation genes, and the development of amyloidosis..
D. Ray, A. Wu, J. E. Wilkinson, H. S. Murphy, Q. Lu, B. Kluve-Beckerman, J. J. Liepnieks, M. Benson, R. Yung, and B. Richardson (2006)
J. Gerontol. A Biol. Sci. Med. Sci.
61, 115-124
|Abstract »|Full Text »|PDF »
DNA Hypermethylation of Promoter of Gene p53 and p16 in Arsenic-Exposed People with and without Malignancy.
S. Chanda, U. B. Dasgupta, D. GuhaMazumder, M. Gupta, U. Chaudhuri, S. Lahiri, S. Das, N. Ghosh, and D. Chatterjee (2006)
Toxicol. Sci.
89, 431-437
|Abstract »|Full Text »|PDF »
Epigenetics and human disease: translating basic biology into clinical applications.
De novo CpG Island Methylation in Human Cancer Cells.
K.-W. Jair, K. E. Bachman, H. Suzuki, A. H. Ting, I. Rhee, R.-W. C. Yen, S. B. Baylin, and K. E. Schuebel (2006)
Cancer Res.
66, 682-692
|Abstract »|Full Text »|PDF »
p66{alpha} and p66{beta} of the Mi-2/NuRD complex mediate MBD2 and histone interaction.
M. Brackertz, Z. Gong, J. Leers, and R. Renkawitz (2006)
Nucleic Acids Res.
34, 397-406
|Abstract »|Full Text »|PDF »
Inhibition of DNA Methyltransferase Activity Prevents Tumorigenesis in a Mouse Model of Prostate Cancer.
M. T. McCabe, J. A. Low, S. Daignault, M. J. Imperiale, K. J. Wojno, and M. L. Day (2006)
Cancer Res.
66, 385-392
|Abstract »|Full Text »|PDF »
Loss of Imprinting of IGF2: A Common Epigenetic Modifier of Intestinal Tumor Risk.
Fractionated Low-Dose Radiation Exposure Leads to Accumulation of DNA Damage and Profound Alterations in DNA and Histone Methylation in the Murine Thymus.
I. Pogribny, I. Koturbash, V. Tryndyak, D. Hudson, S. M.L. Stevenson, O. Sedelnikova, W. Bonner, and O. Kovalchuk (2005)
Mol. Cancer Res.
3, 553-561
|Abstract »|Full Text »|PDF »
Reversal of Hypermethylation and Reactivation of p16INK4a, RAR{beta}, and MGMT Genes by Genistein and Other Isoflavones from Soy.
M. Z. Fang, D. Chen, Y. Sun, Z. Jin, J. K. Christman, and C. S. Yang (2005)
Clin. Cancer Res.
11, 7033-7041
|Abstract »|Full Text »|PDF »
MTHFR Variants Reduce the Risk of G:C->A:T Transition Mutations within the p53 Tumor Suppressor Gene in Colon Tumors.
C. M. Ulrich, K. Curtin, W. Samowitz, J. Bigler, J. D. Potter, B. Caan, and M. L. Slattery (2005)
J. Nutr.
135, 2462-2467
|Abstract »|Full Text »|PDF »
Opposing effects of DNA hypomethylation on intestinal and liver carcinogenesis.
Y. Yamada, L. Jackson-Grusby, H. Linhart, A. Meissner, A. Eden, H. Lin, and R. Jaenisch (2005)
PNAS
102, 13580-13585
|Abstract »|Full Text »|PDF »
In vivo transcriptional regulation of N-Myc target genes is controlled by E-box methylation.
G. Perini, D. Diolaiti, A. Porro, and G. Della Valle (2005)
PNAS
102, 12117-12122
|Abstract »|Full Text »|PDF »
Characterization of DNA Demethylation Effects Induced by 5-Aza-2'-Deoxycytidine in Patients with Myelodysplastic Syndrome.
C. Mund, B. Hackanson, C. Stresemann, M. Lubbert, and F. Lyko (2005)
Cancer Res.
65, 7086-7090
|Abstract »|Full Text »|PDF »
Tumorigenesis: the adaptation of mammalian cells to sustained stress environment by epigenetic alterations and succeeding matched mutations.
Epigenetic Reactivation of Tumor Suppressor Genes by a Novel Small-Molecule Inhibitor of Human DNA Methyltransferases.
B. Brueckner, R. Garcia Boy, P. Siedlecki, T. Musch, H. C. Kliem, P. Zielenkiewicz, S. Suhai, M. Wiessler, and F. Lyko (2005)
Cancer Res.
65, 6305-6311
|Abstract »|Full Text »|PDF »
Comparisons of Brain, Uterus, and Liver mRNA Expression for Cytochrome P450s, DNA Methyltransferase-1, and Catechol-O-Methyltransferase in Prepubertal Female Sprague-Dawley Rats Exposed to a Mixture of Aryl Hydrocarbon Receptor Agonists.
D. Desaulniers, G.-H. Xiao, K. Leingartner, I. Chu, B. Musicki, and B. K. Tsang (2005)
Toxicol. Sci.
86, 175-184
|Abstract »|Full Text »|PDF »
Recruitment of DNA methyltransferase I to DNA repair sites.
O. Mortusewicz, L. Schermelleh, J. Walter, M. C. Cardoso, and H. Leonhardt (2005)
PNAS
102, 8905-8909
|Abstract »|Full Text »|PDF »
Epigenetic and Chromatin Modifiers As Targeted Therapy of Hematologic Malignancies.
MSRE-PCR for analysis of gene-specific DNA methylation.
A. A. Melnikov, R. B. Gartenhaus, A. S. Levenson, N. A. Motchoulskaia, and V. V. Levenson (Chernokhvostov) (2005)
Nucleic Acids Res.
33, e93
|Abstract »|Full Text »|PDF »
Diagnostic and Therapeutic Applications of Epigenetics.
Inactivation of Dnmt3b in Mouse Embryonic Fibroblasts Results in DNA Hypomethylation, Chromosomal Instability, and Spontaneous Immortalization.
J. E. Dodge, M. Okano, F. Dick, N. Tsujimoto, T. Chen, S. Wang, Y. Ueda, N. Dyson, and E. Li (2005)
J. Biol. Chem.
280, 17986-17991
|Abstract »|Full Text »|PDF »
Discovery of Aberrant Expression of R-RAS by Cancer-Linked DNA Hypomethylation in Gastric Cancer Using Microarrays.
M. Nishigaki, K. Aoyagi, I. Danjoh, M. Fukaya, K. Yanagihara, H. Sakamoto, T. Yoshida, and H. Sasaki (2005)
Cancer Res.
65, 2115-2124
|Abstract »|Full Text »|PDF »
Epigenetic Changes in Prostate Cancer: Implication for Diagnosis and Treatment.
L.-C. Li, P. R. Carroll, and R. Dahiya (2005)
J Natl Cancer Inst
97, 103-115
|Abstract »|Full Text »|PDF »
Differential DNA hypermethylation and hypomethylation signatures in colorectal cancer.
J. Frigola, X. Sole, M. F. Paz, V. Moreno, M. Esteller, G. Capella, and M. A. Peinado (2005)
Hum. Mol. Genet.
14, 319-326
|Abstract »|Full Text »|PDF »
