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Mikel Valle,1*Reynald Gillet,2*Sukhjit Kaur,1Anke Henne,3V. Ramakrishnan,2Joachim Frank14
Bacterial ribosomes stalled on defective messenger RNAs
(mRNAs) are rescued by tmRNA, an ~300-nucleotide-long molecule thatfunctions as both transfer RNA (tRNA) and mRNA. Translation thenswitches from the defective message to a short open reading frameon
tmRNA that tags the defective nascent peptide chain for degradation.However, the mechanism by which tmRNA can enter and move throughthe
ribosome is unknown. We present a cryo-electron microscopystudy at
~13 to 15 angstroms of the entry of tmRNA into the ribosome.The
structure reveals how tmRNA could move through the ribosomedespite its
complicated topology and also suggests roles for proteinsS1 and SmpB
in the function of tmRNA.
1 Howard Hughes Medical Institute, Wadsworth
Center, Health Research, Inc., Empire State Plaza, Albany, NY
12201-0509, USA.
2 MRC Laboratory of Molecular
Biology, Hills Road, Cambridge CB2 2QH, UK.
3 Göttingen Genomics Laboratory, Institute of
Microbiology and Genetics, Grisebachstrasse 8, 37077 Göttingen,
Germany.
4 Department of Biomedical Sciences, State
University of New York at Albany, Empire State Plaza, Albany, New York
12201-0509, USA.
*
These authors contributed equally to this work.
To whom correspondence should be addressed.
E-mail: ramak{at}mrc-lmb.cam.ac.uk (V.R.);
joachim{at}wadsworth.org (J.F.)
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