Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 27 February 2003
Science 14 March 2003:
Vol. 299. no. 5613, pp. 1751 - 1753
DOI: 10.1126/science.1080418
Prev | Table of Contents | Next

Reports

Asymmetric Inheritance of Oxidatively Damaged Proteins During Cytokinesis

Hugo Aguilaniu,123 Lena Gustafsson,2 Michel Rigoulet,3 Thomas Nyström1

Carbonylated proteins were visualized in single cells of the budding yeast Saccharomyces cerevisiae, revealing that they accumulate with replicative age. Furthermore, carbonylated proteins were not inherited by daughter cells during cytokinesis. Mother cells of a yeast strain lacking the sir2 gene, a life-span determinant, failed to retain oxidatively damaged proteins during cytokinesis. These findings suggest that a genetically determined, Sir2p-dependent asymmetric inheritance of oxidatively damaged proteins may contribute to free-radical defense and the fitness of newborn cells.

1 Department of Cell and Molecular Biology-Microbiology, Göteborg University, Göteborg, Sweden.
2 Department of Molecular Biotechnology, Chalmers University of Technology, Göteborg, Sweden.
3 Institut de Biochimie et de Génétiques Cellulaires du CNRS, Université Victor Segalen, Bordeaux, France.

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Carbonylated Proteins Are Detectable Only in a Degradation-Resistant Aggregate State in Escherichia coli.
E. Maisonneuve, L. Fraysse, S. Lignon, L. Capron, and S. Dukan (2008)
J. Bacteriol. 190, 6609-6614
   Abstract »    Full Text »    PDF »
Protein Aggregates: an Aging Factor Involved in Cell Death.
E. Maisonneuve, B. Ezraty, and S. Dukan (2008)
J. Bacteriol. 190, 6070-6075
   Abstract »    Full Text »    PDF »
Asymmetric mitosis: Unequal segregation of proteins destined for degradation.
L. C. Fuentealba, E. Eivers, D. Geissert, V. Taelman, and E. M. De Robertis (2008)
PNAS 105, 7732-7737
   Abstract »    Full Text »    PDF »
The malate-aspartate NADH shuttle components are novel metabolic longevity regulators required for calorie restriction-mediated life span extension in yeast.
E. Easlon, F. Tsang, C. Skinner, C. Wang, and S.-J. Lin (2008)
Genes & Dev. 22, 931-944
   Abstract »    Full Text »    PDF »
Bet-hedging and epigenetic inheritance in bacterial cell development.
J.-W. Veening, E. J. Stewart, T. W. Berngruber, F. Taddei, O. P. Kuipers, and L. W. Hamoen (2008)
PNAS 105, 4393-4398
   Abstract »    Full Text »    PDF »
A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy.
I. H. Lee, L. Cao, R. Mostoslavsky, D. B. Lombard, J. Liu, N. E. Bruns, M. Tsokos, F. W. Alt, and T. Finkel (2008)
PNAS 105, 3374-3379
   Abstract »    Full Text »    PDF »
Asymmetric segregation of protein aggregates is associated with cellular aging and rejuvenation.
A. B. Lindner, R. Madden, A. Demarez, E. J. Stewart, and F. Taddei (2008)
PNAS 105, 3076-3081
   Abstract »    Full Text »    PDF »
Slx5 Promotes Transcriptional Silencing and Is Required for Robust Growth in the Absence of Sir2.
R. P. Darst, S. N. Garcia, M. R. Koch, and L. Pillus (2008)
Mol. Cell. Biol. 28, 1361-1372
   Abstract »    Full Text »    PDF »
Metal-catalyzed Oxidation of the Werner Syndrome Protein Causes Loss of Catalytic Activities and Impaired Protein-Protein Interactions.
J. A. Harrigan, J. Piotrowski, L. Di Noto, R. L. Levine, and V. A. Bohr (2007)
J. Biol. Chem. 282, 36403-36411
   Abstract »    Full Text »    PDF »
Yeast mother cell-specific ageing, genetic (in)stability, and the somatic mutation theory of ageing.
P. Laun, C. V. Bruschi, J. Richard Dickinson, M. Rinnerthaler, G. Heeren, R. Schwimbersky, R. Rid, and M. Breitenbach (2007)
Nucleic Acids Res.
   Abstract »    Full Text »    PDF »
Chronological and replicative life-span extension in Saccharomyces cerevisiae by increased dosage of alcohol dehydrogenase 1.
G. Reverter-Branchat, E. Cabiscol, J. Tamarit, M. A. Sorolla, M. Angeles de la Torre, and J. Ros (2007)
Microbiology 153, 3667-3676
   Abstract »    Full Text »    PDF »
Accelerated aging and failure to segregate damaged proteins in Sir2 mutants can be suppressed by overproducing the protein aggregation-remodeling factor Hsp104p.
N. Erjavec, L. Larsson, J. Grantham, and T. Nystrom (2007)
Genes & Dev. 21, 2410-2421
   Abstract »    Full Text »    PDF »
Sir2p-dependent protein segregation gives rise to a superior reactive oxygen species management in the progeny of Saccharomyces cerevisiae.
N. Erjavec and T. Nystrom (2007)
PNAS 104, 10877-10881
   Abstract »    Full Text »    PDF »
The Dihydrolipoamide Acetyltransferase Is a Novel Metabolic Longevity Factor and Is Required for Calorie Restriction-mediated Life Span Extension.
E. Easlon, F. Tsang, I. Dilova, C. Wang, S.-P. Lu, C. Skinner, and S.-J. Lin (2007)
J. Biol. Chem. 282, 6161-6171
   Abstract »    Full Text »    PDF »
Sirtuins in Aging and Disease.
L. Guarente (2007)
Cold Spring Harb Symp Quant Biol 72, 483-488
   Abstract »    PDF »
Aging may be a conditional strategic choice and not an inevitable outcome for bacteria.
M. Watve, S. Parab, P. Jogdand, and S. Keni (2006)
PNAS 103, 14831-14835
   Abstract »    Full Text »    PDF »
Specificity in reactive oxidant signaling: think globally, act locally.
L. S. Terada (2006)
J. Cell Biol. 174, 615-623
   Abstract »    Full Text »    PDF »
Elimination of damaged proteins during differentiation of embryonic stem cells.
M. Hernebring, G. Brolen, H. Aguilaniu, H. Semb, and T. Nystrom (2006)
PNAS 103, 7700-7705
   Abstract »    Full Text »    PDF »
Manganese Is the Link between Frataxin and Iron-Sulfur Deficiency in the Yeast Model of Friedreich Ataxia.
V. Irazusta, E. Cabiscol, G. Reverter-Branchat, J. Ros, and J. Tamarit (2006)
J. Biol. Chem. 281, 12227-12232
   Abstract »    Full Text »    PDF »
Cellular Memory and Hematopoietic Stem Cell Aging.
L. M. Kamminga and G. de Haan (2006)
Stem Cells 24, 1143-1149
   Abstract »    Full Text »    PDF »
Knockout of caspase-like gene, YCA1, abrogates apoptosis and elevates oxidized proteins in Saccharomyces cerevisiae.
M. A. S. Khan, P. B. Chock, and E. R. Stadtman (2005)
PNAS 102, 17326-17331
   Abstract »    Full Text »    PDF »
Metabolism, ubiquinone synthesis, and longevity.
H. Aguilaniu, J. Durieux, and A. Dillin (2005)
Genes & Dev. 19, 2399-2406
   Full Text »    PDF »
Support for the immortal strand hypothesis: neural stem cells partition DNA asymmetrically in vitro.
P. Karpowicz, C. Morshead, A. Kam, E. Jervis, J. Ramunas, V. Cheng, and D. van der Kooy (2005)
J. Cell Biol. 170, 721-732
   Abstract »    Full Text »    PDF »
MiMage: A Pan-European Project on the Role of Mitochondria in Aging.
C. Scheckhuber and H. D. Osiewacz (2005)
Sci. Aging Knowl. Environ. 2005, pe14
   Abstract »    Full Text »
Single-Cell Microbiology: Tools, Technologies, and Applications.
B. F. Brehm-Stecher and E. A. Johnson (2004)
Microbiol. Mol. Biol. Rev. 68, 538-559
   Abstract »    Full Text »    PDF »
Oxidative Damage to Specific Proteins in Replicative and Chronological-aged Saccharomyces cerevisiae: COMMON TARGETS AND PREVENTION BY CALORIE RESTRICTION.
G. Reverter-Branchat, E. Cabiscol, J. Tamarit, and J. Ros (2004)
J. Biol. Chem. 279, 31983-31989
   Abstract »    Full Text »    PDF »
Biological detection of low radiation doses by combining results of two microarray analysis methods.
G. Mercier, N. Berthault, J. Mary, J. Peyre, A. Antoniadis, J.-P. Comet, A. Cornuejols, C. Froidevaux, and M. Dutreix (2004)
Nucleic Acids Res. 32, e12
   Abstract »    Full Text »    PDF »
Plasmid Accumulation Reduces Life Span in Saccharomyces cerevisiae.
A. A. Falcon and J. P. Aris (2003)
J. Biol. Chem. 278, 41607-41617
   Abstract »    Full Text »    PDF »
An Age-Induced Switch to a Hyper-Recombinational State.
M. A. McMurray and D. E. Gottschling (2003)
Science 301, 1908-1911
   Abstract »    Full Text »    PDF »
Longevity Regulation in Saccharomyces cerevisiae: Linking Metabolism, Genome Stability, and Heterochromatin.
K. J. Bitterman, O. Medvedik, and D. A. Sinclair (2003)
Microbiol. Mol. Biol. Rev. 67, 376-399
   Abstract »    Full Text »    PDF »
Senescence in a Bacterium with Asymmetric Division.
M. Ackermann, S. C. Stearns, and U. Jenal (2003)
Science 300, 1920
   Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)