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Originally published in Science Express on 14 November 2002
Science 13 December 2002:
Vol. 298. no. 5601, pp. 2195 - 2199
DOI: 10.1126/science.1077642
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Reports

Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation

Paul Wentworth Jr.,1 Jonathan E. McDunn,1 Anita D. Wentworth,1 Cindy Takeuchi,2 Jorge Nieva,3 Teresa Jones,1 Cristina Bautista,1 Julie M. Ruedi,3 Abel Gutierrez,3 Kim D. Janda,1 Bernard M. Babior,3 Albert Eschenmoser,14 Richard A. Lerner1

Recently, we showed that antibodies catalyze the generation of hydrogen peroxide (H2O2) from singlet molecular oxygen (1O2*) and water. Here, we show that this process can lead to efficient killing of bacteria, regardless of the antigen specificity of the antibody. H2O2 production by antibodies alone was found to be not sufficient for bacterial killing. Our studies suggested that the antibody-catalyzed water-oxidation pathway produced an additional molecular species with a chemical signature similar to that of ozone. This species is also generated during the oxidative burst of activated human neutrophils and during inflammation. These observations suggest that alternative pathways may exist for biological killing of bacteria that are mediated by potent oxidants previously unknown to biology.

1 Department of Chemistry,
2 Department of Immunology,
3 Department of Molecular and Experimental Medicine and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
4 Laboratorium für organische Chemie, Eidgenössichsche Technische Hochschule (ETH) Hönggerberg HCl-H309, Universitaetstrasse 16 CH-8093 Zürich, Switzerland.

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