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Science 6 December 2002:
Vol. 298. no. 5600, pp. 1999 - 2002
DOI: 10.1126/science.1074632
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Reports

Regulation of Spermatogenesis by Testis-Specific, Cytoplasmic Poly(A) Polymerase TPAP

Shin-ichi Kashiwabara,1 Junko Noguchi,3 Tiangang Zhuang,1 Ko Ohmura,1 Arata Honda,1 Shin Sugiura,1 Kiyoko Miyamoto,1 Satoru Takahashi,2 Kimiko Inoue,4 Atsuo Ogura,4 Tadashi Baba1*

Spermatogenesis is a highly specialized process of cellular differentiation to produce spermatozoa. This differentiation process accompanies morphological changes that are controlled by a number of genes expressed in a stage-specific manner during spermatogenesis. Here we show that in mice, the absence of a testis-specific, cytoplasmic polyadenylate [poly(A)] polymerase, TPAP, results in the arrest of spermiogenesis. TPAP-deficient mice display impaired expression of haploid-specific genes that are required for the morphogenesis of germ cells. The TPAP deficiency also causes incomplete elongation of poly(A) tails of particular transcription factor messenger RNAs. Although the overall cellular level of the transcription factor TAF10 is unaffected, TAF10 is insufficiently transported into the nucleus of germ cells. We propose that TPAP governs germ cell morphogenesis by modulating specific transcription factors at posttranscriptional and posttranslational levels.

1 Institute of Applied Biochemistry,
2 Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan.
3 Germ Cell Conservation Laboratory, National Institute of Agrobiological Resources, Tsukuba Science City, Ibaraki 305-8602, Japan.
4 Bioresource Center, The Institute of Physical and Chemical Research (RIKEN), Tsukuba Science City, Ibaraki 305-0074, Japan.
*   To whom correspondence should be addressed. E-mail: acroman{at}sakura.cc.tsukuba.ac.jp

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Science. ISSN 0036-8075 (print), 1095-9203 (online)