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Science 22 November 2002:
Vol. 298. no. 5598, pp. 1600 - 1602
DOI: 10.1126/science.1077353
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Reports

Fluorometric Detection of Enzyme Activity with Synthetic Supramolecular Pores

Gopal Das, Pinaki Talukdar, Stefan Matile*

The reversible blockage of synthetic pores formed by rigid-rod beta  barrels, either by substrates or products, was used to sense a variety of enzymatic reactions in high-throughput format with "naked-eye" fluorescent detection. Improvement of sensor sensitivity beyond three orders of magnitude by straightforward internal mutations underscores the functional plasticity of rigid-rod beta  barrels. Such detectors of enzyme activity with the aforementioned characteristics are needed in areas as diverse as proteomics and environmentally benign organic synthesis.

Department of Organic Chemistry, University of Geneva, CH-1211 Geneva, Switzerland.
*   To whom correspondence should be addressed. E-mail: stefan.matile{at}chiorg.unige.ch

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Enzyme screening with synthetic multifunctional pores: Focus on biopolymers.
N. Sorde, G. Das, and S. Matile (2003)
PNAS 100, 11964-11969
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