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Science 1 November 2002:
Vol. 298. no. 5595, pp. 1023 - 1025
DOI: 10.1126/science.1074675
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Reports

Microbial Dehalorespiration with 1,1,1-Trichloroethane

Baolin Sun,1 Benjamin M. Griffin,12 Héctor L. Ayala-del-Río,12 Syed A. Hashsham,13 James M. Tiedje12*

1,1,1-Trichloroethane (TCA) is a ubiquitous environmental pollutant because of its widespread use as an industrial solvent, its improper disposal, and its substantial emission to the atmosphere. We report the isolation of an anaerobic bacterium, strain TCA1, that reductively dechlorinates TCA to 1,1-dichloroethane and chloroethane. Strain TCA1 required H2 as an electron donor and TCA as an electron acceptor for growth, indicating that dechlorination is a respiratory process. Phylogenetic analysis indicated that strain TCA1 is related to gram-positive bacteria with low DNA G+C content and that its closest relative is Dehalobacter restrictus, an obligate H2-oxidizing, chloroethene-respiring bacterium.

1 Center for Microbial Ecology,
2 Department of Microbiology and Molecular Genetics,
3 Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824-1325, USA.
*   To whom correspondence should be addressed. E-mail: tiedjej{at}msu.edu

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Characterization of a Dehalobacter Coculture That Dechlorinates 1,2-Dichloroethane to Ethene and Identification of the Putative Reductive Dehalogenase Gene.
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A Novel Dehalobacter Species Is Involved in Extensive 4,5,6,7-Tetrachlorophthalide Dechlorination.
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The Metabolism and Dechlorination of Chlorotyrosine in Vivo.
A. R. Mani, S. Ippolito, J. C. Moreno, T. J. Visser, and K. P. Moore (2007)
J. Biol. Chem. 282, 29114-29121
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A 1,1,1-Trichloroethane-Degrading Anaerobic Mixed Microbial Culture Enhances Biotransformation of Mixtures of Chlorinated Ethenes and Ethanes.
A. Grostern and E. A. Edwards (2006)
Appl. Envir. Microbiol. 72, 7849-7856
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Growth of Dehalobacter and Dehalococcoides spp. during Degradation of Chlorinated Ethanes.
A. Grostern and E. A. Edwards (2006)
Appl. Envir. Microbiol. 72, 428-436
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Stereoselective Microbial Dehalorespiration with Vicinal Dichlorinated Alkanes.
S. De Wildeman, G. Diekert, H. Van Langenhove, and W. Verstraete (2003)
Appl. Envir. Microbiol. 69, 5643-5647
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