Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Johnson & Johnson

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 15 August 2002
Science 18 October 2002:
Vol. 298. no. 5593, pp. 608 - 611
DOI: 10.1126/science.1075901
Prev | Table of Contents | Next

Reports

Role of Predicted Metalloprotease Motif of Jab1/Csn5 in Cleavage of Nedd8 from Cul1

Gregory A. Cope,1 Greg S. B. Suh,3* L. Aravind,4 Sylvia E. Schwarz,1dagger S. Lawrence Zipursky,23 Eugene V. Koonin,4 Raymond J. Deshaies12ddagger

COP9 signalosome (CSN) cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. The Jab1/MPN domain metalloenzyme (JAMM) motif in the Jab1/Csn5 subunit was found to underlie CSN's Nedd8 isopeptidase activity. JAMM is found in proteins from archaea, bacteria, and eukaryotes, including the Rpn11 subunit of the 26S proteasome. Metal chelators and point mutations within JAMM abolished CSN-dependent cleavage of Nedd8 from Cul1, yet had little effect on CSN complex assembly. Optimal SCF activity in yeast and both viability and proper photoreceptor cell (R cell) development in Drosophila melanogaster required an intact Csn5 JAMM domain. We propose that JAMM isopeptidases play important roles in a variety of physiological pathways.

1 Department of Biology, California Institute of Technology (CalTech), Pasadena, CA 91125, USA.
2 Howard Hughes Medical Institute,
3 Department of Biological Chemistry, The School of Medicine, University of California at Los Angeles (UCLA), Los Angeles, CA 90095, USA.
4 National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
*   Present address: Department of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

dagger    Present address: G2M Cancer Drugs AG, Frankfurt/Main, Germany.

ddagger    To whom correspondence should be addressed. E-mail: deshaies{at}caltech.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
DEN1 deneddylates non-cullin proteins in vivo.
Y. Chan, J. Yoon, J.-T. Wu, H.-J. Kim, K.-T. Pan, J. Yim, and C.-T. Chien (2008)
J. Cell Sci. 121, 3218-3223
   Abstract »    Full Text »    PDF »
Auxin Responses in Mutants of the Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC9 Signalosome.
E. M. N. Dohmann, M. P. Levesque, E. Isono, M. Schmid, and C. Schwechheimer (2008)
Plant Physiology 147, 1369-1379
   Abstract »    Full Text »    PDF »
Genetic analysis of CAND1-CUL1 interactions in Arabidopsis supports a role for CAND1-mediated cycling of the SCFTIR1 complex.
W. Zhang, H. Ito, M. Quint, H. Huang, L. D. Noel, and W. M. Gray (2008)
PNAS 105, 8470-8475
   Abstract »    Full Text »    PDF »
The COP9/Signalosome Increases the Efficiency of von Hippel-Lindau Protein Ubiquitin Ligase-mediated Hypoxia-inducible Factor-{alpha} Ubiquitination.
Y. Miyauchi, M. Kato, F. Tokunaga, and K. Iwai (2008)
J. Biol. Chem. 283, 16622-16631
   Abstract »    Full Text »    PDF »
Targeted inactivation of the COP9 signalosome impairs multiple stagesof T cell development.
M. Panattoni, F. Sanvito, V. Basso, C. Doglioni, G. Casorati, E. Montini, J. R. Bender, A. Mondino, and R. Pardi (2008)
J. Exp. Med. 205, 465-477
   Abstract »    Full Text »    PDF »
CSN5 Isopeptidase Activity Links COP9 Signalosome Activation to Breast Cancer Progression.
A. S. Adler, L. E. Littlepage, M. Lin, T. L.A. Kawahara, D. J. Wong, Z. Werb, and H. Y. Chang (2008)
Cancer Res. 68, 506-515
   Abstract »    Full Text »    PDF »
CIF-1, a Shared Subunit of the COP9/Signalosome and Eukaryotic Initiation Factor 3 Complexes, Regulates MEL-26 Levels in the Caenorhabditis elegans Embryo.
S. Luke-Glaser, M. Roy, B. Larsen, T. Le Bihan, P. Metalnikov, M. Tyers, M. Peter, and L. Pintard (2007)
Mol. Cell. Biol. 27, 4526-4540
   Abstract »    Full Text »    PDF »
An eight-subunit COP9 signalosome with an intact JAMM motif is required for fungal fruit body formation.
S. Busch, E. U. Schwier, K. Nahlik, O. Bayram, K. Helmstaedt, O. W. Draht, S. Krappmann, O. Valerius, W. N. Lipscomb, and G. H. Braus (2007)
PNAS 104, 8089-8094
   Abstract »    Full Text »    PDF »
Ubiquitin, Hormones and Biotic Stress in Plants.
K. Dreher and J. Callis (2007)
Ann. Bot. 99, 787-822
   Abstract »    Full Text »    PDF »
The Subunit CSN6 of the COP9 Signalosome Is Cleaved during Apoptosis.
J. d. S. Correia, Y. Miranda, N. Leonard, and R. J. Ulevitch (2007)
J. Biol. Chem. 282, 12557-12565
   Abstract »    Full Text »    PDF »
Characterization of the VIER F-BOX PROTEINE Genes from Arabidopsis Reveals Their Importance for Plant Growth and Development.
K. M. Schwager, L. I. A. Calderon-Villalobos, E. M.N. Dohmann, B. C. Willige, S. Knierer, C. Nill, and C. Schwechheimer (2007)
PLANT CELL 19, 1163-1178
   Abstract »    Full Text »    PDF »
The Cullin3 Ubiquitin Ligase Functions as a Nedd8-bound Heterodimer.
W. Wimuttisuk and J. D. Singer (2007)
Mol. Biol. Cell 18, 899-909
   Abstract »    Full Text »    PDF »
The Assembly Pathway of the 19S Regulatory Particle of the Yeast 26S Proteasome.
E. Isono, K. Nishihara, Y. Saeki, H. Yashiroda, N. Kamata, L. Ge, T. Ueda, Y. Kikuchi, K. Tanaka, A. Nakano, et al. (2007)
Mol. Biol. Cell 18, 569-580
   Abstract »    Full Text »    PDF »
RIG-G as a key mediator of the antiproliferative activity of interferon-related pathways through enhancing p21 and p27 proteins.
S. Xiao, D. Li, H.-Q. Zhu, M.-G. Song, X.-R. Pan, P.-M. Jia, L.-L. Peng, A.-X. Dou, G.-Q. Chen, S.-J. Chen, et al. (2006)
PNAS 103, 16448-16453
   Abstract »    Full Text »    PDF »
The COP9 Signalosome Regulates Skp2 Levels and Proliferation of Human Cells.
S. Denti, M. E. Fernandez-Sanchez, L. Rogge, and E. Bianchi (2006)
J. Biol. Chem. 281, 32188-32196
   Abstract »    Full Text »    PDF »
Jab1 Mediates Cytoplasmic Localization and Degradation of West Nile Virus Capsid Protein.
W. Oh, M.-R. Yang, E.-W. Lee, K.-m. Park, S. Pyo, J.-s. Yang, H.-W. Lee, and J. Song (2006)
J. Biol. Chem. 281, 30166-30174
   Abstract »    Full Text »    PDF »
SUMO-Conjugating and SUMO-Deconjugating Enzymes from Arabidopsis.
T. Colby, A. Matthai, A. Boeckelmann, and H.-P. Stuible (2006)
Plant Physiology 142, 318-332
   Abstract »    Full Text »    PDF »
Jab1 Induces the Cytoplasmic Localization and Degradation of p53 in Coordination with Hdm2.
W. Oh, E.-W. Lee, Y. H. Sung, M.-R. Yang, J. Ghim, H.-W. Lee, and J. Song (2006)
J. Biol. Chem. 281, 17457-17465
   Abstract »    Full Text »    PDF »
The Ubiquitin Isopeptidase UBPY Regulates Endosomal Ubiquitin Dynamics and Is Essential for Receptor Down-regulation.
P. E. Row, I. A. Prior, J. McCullough, M. J. Clague, and S. Urbe (2006)
J. Biol. Chem. 281, 12618-12624
   Abstract »    Full Text »    PDF »
Cul4A and DDB1 Associate with Skp2 To Target p27Kip1 for Proteolysis Involving the COP9 Signalosome..
T. Bondar, A. Kalinina, L. Khair, D. Kopanja, A. Nag, S. Bagchi, and P. Raychaudhuri (2006)
Mol. Cell. Biol. 26, 2531-2539
   Abstract »    Full Text »    PDF »
CAND1-Mediated Substrate Adaptor Recycling Is Required for Efficient Repression of Nrf2 by Keap1.
S.-C. Lo and M. Hannink (2006)
Mol. Cell. Biol. 26, 1235-1244
   Abstract »    Full Text »    PDF »
Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p.
P. BELLARE, A. K. KUTACH, A. K. RINES, C. GUTHRIE, and E. J. SONTHEIMER (2006)
RNA 12, 292-302
   Abstract »    Full Text »    PDF »
The S100A7-c-Jun Activation Domain Binding Protein 1 Pathway Enhances Prosurvival Pathways in Breast Cancer.
E. D. Emberley, Y. Niu, L. Curtis, S. Troup, S. K. Mandal, J. N. Myers, S. B. Gibson, L. C. Murphy, and P. H. Watson (2005)
Cancer Res. 65, 5696-5702
   Abstract »    Full Text »    PDF »
Loss of the CONSTITUTIVE PHOTOMORPHOGENIC9 Signalosome Subunit 5 Is Sufficient to Cause the cop/det/fus Mutant Phenotype in Arabidopsis.
E. M.N. Dohmann, C. Kuhnle, and C. Schwechheimer (2005)
PLANT CELL 17, 1967-1978
   Abstract »    Full Text »    PDF »
The COP9 signalosome regulates the Neurospora circadian clock by controlling the stability of the SCFFWD-1 complex.
Q. He, P. Cheng, Q. He, and Y. Liu (2005)
Genes & Dev. 19, 1518-1531
   Abstract »    Full Text »    PDF »
The Jab1/COP9 signalosome subcomplex is a downstream mediator of Bcr-Abl kinase activity and facilitates cell-cycle progression.
K. Tomoda, J.-y. Kato, E. Tatsumi, T. Takahashi, Y. Matsuo, and N. Yoneda-Kato (2005)
Blood 105, 775-783
   Abstract »    Full Text »    PDF »
The Ubiquitin-Proteasome Pathway and Plant Development.
J. Moon, G. Parry, and M. Estelle (2004)
PLANT CELL 16, 3181-3195
   Full Text »    PDF »
Translational Regulation via 5' mRNA Leader Sequences Revealed by Mutational Analysis of the Arabidopsis Translation Initiation Factor Subunit eIF3h.
T.-H. Kim, B.-H. Kim, A. Yahalom, D. A. Chamovitz, and A. G. von Arnim (2004)
PLANT CELL 16, 3341-3356
   Abstract »    Full Text »    PDF »
Multiple Functions of Jab1 Are Required for Early Embryonic Development and Growth Potential in Mice.
K. Tomoda, N. Yoneda-Kato, A. Fukumoto, S. Yamanaka, and J.-y. Kato (2004)
J. Biol. Chem. 279, 43013-43018
   Abstract »    Full Text »    PDF »
AMSH is an endosome-associated ubiquitin isopeptidase.
J. McCullough, M. J. Clague, and S. Urbe (2004)
J. Cell Biol. 166, 487-492
   Abstract »    Full Text »    PDF »
Interaction between Glucose-regulated Destruction Domain of DNA Topoisomerase II{alpha} and MPN Domain of Jab1/CSN5.
J. Yun, A. Tomida, T. Andoh, and T. Tsuruo (2004)
J. Biol. Chem. 279, 31296-31303
   Abstract »    Full Text »    PDF »
Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation.
S. Feng, Y. Shen, J. A. Sullivan, V. Rubio, Y. Xiong, T.-p. Sun, and X. W. Deng (2004)
PLANT CELL 16, 1870-1882
   Abstract »    Full Text »    PDF »
Arabidopsis ETA2, an Apparent Ortholog of the Human Cullin-Interacting Protein CAND1, Is Required for Auxin Responses Mediated by the SCFTIR1 Ubiquitin Ligase.
H.-w. Chuang, W. Zhang, and W. M. Gray (2004)
PLANT CELL 16, 1883-1897
   Abstract »    Full Text »    PDF »
A Genomic Analysis of Rat Proteases and Protease Inhibitors.
X. S. Puente and C. Lopez-Otin (2004)
Genome Res. 14, 609-622
   Abstract »    Full Text »    PDF »
Similar active sites in lysostaphins and D-Ala-D-Ala metallopeptidases.
M. Bochtler, S. G. Odintsov, M. Marcyjaniak, and I. Sabala (2004)
Protein Sci. 13, 854-861
   Abstract »    Full Text »    PDF »
Complementary Roles for Rpn11 and Ubp6 in Deubiquitination and Proteolysis by the Proteasome.
A. Guterman and M. H. Glickman (2004)
J. Biol. Chem. 279, 1729-1738
   Abstract »    Full Text »    PDF »
Specific and Covalent Targeting of Conjugating and Deconjugating Enzymes of Ubiquitin-Like Proteins.
J. Hemelaar, A. Borodovsky, B. M. Kessler, D. Reverter, J. Cook, N. Kolli, T. Gan-Erdene, K. D. Wilkinson, G. Gill, C. D. Lima, et al. (2004)
Mol. Cell. Biol. 24, 84-95
   Abstract »    Full Text »    PDF »
Encore facilitates SCF-Ubiquitin-proteasome-dependent proteolysis during Drosophila oogenesis.
J. T. Ohlmeyer and T. Schupbach (2003)
Development 130, 6339-6349
   Abstract »    Full Text »    PDF »
Disruption of the COP9 Signalosome Csn2 Subunit in Mice Causes Deficient Cell Proliferation, Accumulation of p53 and Cyclin E, and Early Embryonic Death.
K. Lykke-Andersen, L. Schaefer, S. Menon, X.-W. Deng, J. B. Miller, and N. Wei (2003)
Mol. Cell. Biol. 23, 6790-6797
   Abstract »    Full Text »    PDF »
COP9 Signalosome Subunit 3 Is Essential for Maintenance of Cell Proliferation in the Mouse Embryonic Epiblast.
J. Yan, K. Walz, H. Nakamura, S. Carattini-Rivera, Q. Zhao, H. Vogel, N. Wei, M. J. Justice, A. Bradley, and J. R. Lupski (2003)
Mol. Cell. Biol. 23, 6798-6808
   Abstract »    Full Text »    PDF »
Use of RNA Interference and Complementation To Study the Function of the Drosophila and Human 26S Proteasome Subunit S13.
J. Lundgren, P. Masson, C. A. Realini, and P. Young (2003)
Mol. Cell. Biol. 23, 5320-5330
   Abstract »    Full Text »    PDF »
DEN1 Is a Dual Function Protease Capable of Processing the C Terminus of Nedd8 and Deconjugating Hyper-neddylated CUL1.
K. Wu, K. Yamoah, G. Dolios, T. Gan-Erdene, P. Tan, A. Chen, C.-g. Lee, N. Wei, K. D. Wilkinson, R. Wang, et al. (2003)
J. Biol. Chem. 278, 28882-28891
   Abstract »    Full Text »    PDF »
Identification and Characterization of DEN1, a Deneddylase of the ULP Family.
T. Gan-Erdene, K. Nagamalleswari, L. Yin, K. Wu, Z.-Q. Pan, and K. D. Wilkinson (2003)
J. Biol. Chem. 278, 28892-28900
   Abstract »    Full Text »    PDF »
NEDP1, a Highly Conserved Cysteine Protease That deNEDDylates Cullins.
H. M. Mendoza, L.-n. Shen, C. Botting, A. Lewis, J. Chen, B. Ink, and R. T. Hay (2003)
J. Biol. Chem. 278, 25637-25643
   Abstract »    Full Text »    PDF »
A Novel Type of Deubiquitinating Enzyme.
P. C. Evans, T. S. Smith, M.-J. Lai, M. G. Williams, D. F. Burke, K. Heyninck**, M. M. Kreike, R. Beyaert, T. L. Blundell, and P. J. Kilshaw (2003)
J. Biol. Chem. 278, 23180-23186
   Abstract »    Full Text »    PDF »
Cop9/signalosome subunits and Pcu4 regulate ribonucleotide reductase by both checkpoint-dependent and -independent mechanisms.
C. Liu, K. A. Powell, K. Mundt, L. Wu, A. M. Carr, and T. Caspari (2003)
Genes & Dev. 17, 1130-1140
   Abstract »    Full Text »    PDF »
The COP9 Signalosome Interacts with SCFUFO and Participates in Arabidopsis Flower Development.
X. Wang, S. Feng, N. Nakayama, W. L. Crosby, V. Irish, X. W. Deng, and N. Wei (2003)
PLANT CELL 15, 1071-1082
   Abstract »    Full Text »
The COP9 Signalosome Interacts Physically with SCFCOI1 and Modulates Jasmonate Responses.
S. Feng, L. Ma, X. Wang, D. Xie, S. P. Dinesh-Kumar, N. Wei, and X. W. Deng (2003)
PLANT CELL 15, 1083-1094
   Abstract »    Full Text »
Characterization of the Last Subunit of the Arabidopsis COP9 Signalosome: Implications for the Overall Structure and Origin of the Complex.
G. Serino, H. Su, Z. Peng, T. Tsuge, N. Wei, H. Gu, and X. W. Deng (2003)
PLANT CELL 15, 719-731
   Abstract »    Full Text »    PDF »
Role of Rpn11 Metalloprotease in Deubiquitination and Degradation by the 26S Proteasome.
R. Verma, L. Aravind, R. Oania, W. H. McDonald, J. R. Yates III, E. V. Koonin, and R. J. Deshaies (2002)
Science 298, 611-615
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)