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Modulation of Postendocytic Sorting of G Protein-Coupled Receptors
Jennifer L. Whistler,1*Johan Enquist,2Aaron Marley,3Jamie Fong,1Fredrik Gladher,2Pamela Tsuruda,3Stephen R. Murray,3Mark von Zastrow3*
Recycling of the mu opioid receptor to the plasma membrane after
endocytosis promotes rapid resensitization of signal transduction,whereas targeting of the delta opioid receptor (DOR) to lysosomescauses proteolytic down-regulation. We identified a protein thatbinds
preferentially to the cytoplasmic tail of the DOR as a candidateheterotrimeric GTP-binding protein (G protein)-coupled
receptor-associatedsorting protein (GASP). Disruption of the DOR-GASP
interactionthrough receptor mutation or overexpression of a dominant
negativefragment of GASP inhibited receptor trafficking to lysosomes
andpromoted recycling. The GASP family of proteins may modulate
lysosomalsorting and functional down-regulation of a variety of G
protein-coupledreceptors.
1 Department of Neurology, Ernest Gallo Clinic
and Research Center, University of California, San Francisco,
Emeryville, CA 94608, USA.
2 Program in Molecular
Medicine, University of Lund, Sweden.
3 Program in
Cell Biology, Department of Psychiatry and Department of Cellular and
Molecular Pharmacology, University of California, San Francisco, CA
94143, USA.
*
To whom correspondence should be addressed. E-mail:
shooz2{at}itsa.ucsf.edu (J.L.W); zastrow{at}itsa.ucsf.edu (M.v.Z.)
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Ubiquitination-independent Trafficking of G Protein-coupled Receptors to Lysosomes.
