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Science 12 April 2002:
Vol. 296. no. 5566, pp. 343 - 346
DOI: 10.1126/science.1068181
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Reports

A MADS-Box Gene Necessary for Fruit Ripening at the Tomato Ripening-Inhibitor (Rin) Locus

Julia Vrebalov,12 Diane Ruezinsky,2 Veeraragavan Padmanabhan,2 Ruth White,12 Diana Medrano,12 Rachel Drake,3 Wolfgang Schuch,3 Jim Giovannoni1*

Tomato plants harboring the ripening-inhibitor (rin) mutation yield fruits that fail to ripen. Additionally, rin plants display enlarged sepals and loss of inflorescence determinacy. Positional cloning of the rin locus revealed two tandem MADS-box genes (LeMADS-RIN and LeMADS-MC), whose expression patterns suggested roles in fruit ripening and sepal development, respectively. The rin mutation alters expression of both genes. Gene repression and mutant complementation demonstrate that LeMADS-RIN regulates ripening, whereas LeMADS-MC affects sepal development and inflorescence determinacy. LeMADS-RIN demonstrates an agriculturally important function of plant MADS-box genes and provides molecular insight into nonhormonal (developmental) regulation of ripening.

1 U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) Plant, Soil and Nutrition Lab and Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, USA.
2 Texas A&M University, Department of Horticultural Sciences, College Station, TX 77843, USA.
3 Zeneca Plant Sciences (Syngenta), Jeallots Hill Research Station, Bracknell, Berkshire RG12 6EY, UK.
*   To whom correspondence should be addressed. E-mail: jjg33{at}cornell.edu

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