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T Cell Receptor Signaling Precedes Immunological Synapse Formation
Kyeong-Hee Lee,1Amy D. Holdorf,1Michael L. Dustin,2Andrew C. Chan,3Paul M. Allen,1Andrey S. Shaw1*
The area of contact between a T cell and an
antigen-presenting cell (APC) is known as the immunological synapse.
Althoughits exact function is unknown, one model suggests that it
allowsfor T cell receptor (TCR) clustering and for sustained
signalingin T cells for many hours. Here we demonstrate that
TCR-mediatedtyrosine kinase signaling in naïve T cells
occurred primarilyat the periphery of the synapse and was largely
abated beforemature immunological synapses had formed. These data
suggest thatmany hours of TCR signaling are not required for T cell
activation.These observations challenge current ideas about the role
of immunologicalsynapses in T cell activation.
1 Department of Pathology and Immunology,
Washington University School of Medicine, 660 South Euclid, Box 8118, Saint Louis, MO 63110, USA.
2 Program in Molecular
Pathogenesis, Skirball Institute of Biomolecular Medicine, New York
University, 540 First Avenue, New York, NY 10016, USA.
3 Genentech, Department of Immunology, 530 Forbes
Boulevard, One DNA Way, South San Francisco, CA 94080, USA.
*
To whom correspondence should be addressed. E-mail:
shaw{at}pathbox.wustl.edu
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