Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Originally published in Science Express on 17 January 2002
Science 22 February 2002:
Vol. 295. no. 5559, pp. 1536 - 1539
DOI: 10.1126/science.1064913
Prev | Table of Contents | Next

Reports

Retrograde Support of Neuronal Survival Without Retrograde Transport of Nerve Growth Factor

Bronwyn L. MacInnis, Robert B. Campenot*

Application of nerve growth factor (NGF) covalently cross-linked to beads increased the phosphorylation of TrkA and Akt, but not of mitogen-activated protein kinase, in cultured rat sympathetic neurons. NGF beads or iodine-125-labeled NGF beads supplied to distal axons resulted in the survival of over 80% of the neurons for 30 hours, with little or no retrograde transport of iodine-125-labeled NGF; whereas application of free iodine-125-labeled NGF (0.5 nanograms per milliliter) produced 20-fold more retrograde transport, but only 29% of the neurons survived. Thus, in contrast to widely accepted theory, a neuronal survival signal can reach the cell bodies unaccompanied by the NGF that initiated it.

Department of Cell Biology, 6-14 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7.
*   To whom correspondence should be addressed. E-mail: bob.campenot{at}ualberta.ca

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Proneurotrophin-3 Is a Neuronal Apoptotic Ligand: Evidence for Retrograde-Directed Cell Killing.
H. Yano, R. Torkin, L. A. Martin, M. V. Chao, and K. K. Teng (2009)
J. Neurosci. 29, 14790-14802
   Abstract »    Full Text »    PDF »
Spred2 interaction with the late endosomal protein NBR1 down-regulates fibroblast growth factor receptor signaling.
F. K. Mardakheh, M. Yekezare, L. M. Machesky, and J. K. Heath (2009)
J. Cell Biol. 187, 265-277
   Abstract »    Full Text »    PDF »
Cell Replacement Therapy in the Inner Ear: Implications for Cochlear Implantation.
K. A. Belzner and B. M. Ryals (2009)
Hearing and Hearing Disorders in Childhood 19, 98-107
   Abstract »    Full Text »    PDF »
Transthyretin Internalization by Sensory Neurons Is Megalin Mediated and Necessary for Its Neuritogenic Activity.
C. E. Fleming, F. M. Mar, F. Franquinho, M. J. Saraiva, and M. M. Sousa (2009)
J. Neurosci. 29, 3220-3232
   Abstract »    Full Text »    PDF »
Glial Cells as Players in Parkinsonism: The "Good," the "Bad," and the "Mysterious" Glia.
M. A. Mena and J. Garcia de Yebenes (2008)
Neuroscientist 14, 544-560
   Abstract »    PDF »
Clathrin-dependent Endocytosis Is Required for TrkB-dependent Akt-mediated Neuronal Protection and Dendritic Growth.
J. Zheng, W.-H. Shen, T.-J. Lu, Y. Zhou, Q. Chen, Z. Wang, T. Xiang, Y.-C. Zhu, C. Zhang, S. Duan, et al. (2008)
J. Biol. Chem. 283, 13280-13288
   Abstract »    Full Text »    PDF »
One at a time, live tracking of NGF axonal transport using quantum dots.
B. Cui, C. Wu, L. Chen, A. Ramirez, E. L. Bearer, W.-P. Li, W. C. Mobley, and S. Chu (2007)
PNAS 104, 13666-13671
   Abstract »    Full Text »    PDF »
Unraveling the Story of NGF-mediated Sensitization of Nociceptive Sensory Neurons: ON or OFF the Trks?.
G. D. Nicol and M. R. Vasko (2007)
Mol. Interv. 7, 26-41
   Abstract »    Full Text »    PDF »
Dependence of neurotrophic factor activation of Trk tyrosine kinase receptors on cellular sialidase.
A. Woronowicz, S. R. Amith, K. De Vusser, W. Laroy, R. Contreras, S. Basta, and M. R. Szewczuk (2007)
Glycobiology 17, 10-24
   Abstract »    Full Text »    PDF »
Spatially distinct and functionally independent mechanisms of axonal degeneration in a model of HIV-associated sensory neuropathy.
G. Melli, S. C. Keswani, A. Fischer, W. Chen, and A. Hoke (2006)
Brain 129, 1330-1338
   Abstract »    Full Text »    PDF »
Pincher-Mediated Macroendocytosis Underlies Retrograde Signaling by Neurotrophin Receptors.
G. Valdez, W. Akmentin, P. Philippidou, R. Kuruvilla, D. D. Ginty, and S. Halegoua (2005)
J. Neurosci. 25, 5236-5247
   Abstract »    Full Text »    PDF »
Trafficking of a Ligand-Receptor Complex on the Growth Cones as an Essential Step for the Uptake of Nerve Growth Factor at the Distal End of the Axon: A Single-Molecule Analysis.
T. Tani, Y. Miyamoto, K. E. Fujimori, T. Taguchi, T. Yanagida, Y. Sako, and Y. Harada (2005)
J. Neurosci. 25, 2181-2191
   Abstract »    Full Text »    PDF »
TRKing Signals Through the Golgi.
A. Sorkin (2005)
Sci. STKE 2005, pe1
   Abstract »    Full Text »    PDF »
Transactivation of Trk Neurotrophin Receptors by G-Protein-Coupled Receptor Ligands Occurs on Intracellular Membranes.
R. Rajagopal, Z.-Y. Chen, F. S. Lee, and M. V. Chao (2004)
J. Neurosci. 24, 6650-6658
   Abstract »    Full Text »    PDF »
Rapid Axoglial Signaling Mediated by Neuregulin and Neurotrophic Factors.
R. M. Esper and J. A. Loeb (2004)
J. Neurosci. 24, 6218-6227
   Abstract »    Full Text »    PDF »
Glial Lipoproteins Stimulate Axon Growth of Central Nervous System Neurons in Compartmented Cultures.
H. Hayashi, R. B. Campenot, D. E. Vance, and J. E. Vance (2004)
J. Biol. Chem. 279, 14009-14015
   Abstract »    Full Text »    PDF »
Organization and translation of mRNA in sympathetic axons.
S.-K. Lee and P. J. Hollenbeck (2003)
J. Cell Sci. 116, 4467-4478
   Abstract »    Full Text »    PDF »
Individual and Combined Effects of TrkA and p75NTR Nerve Growth Factor Receptors: A ROLE FOR THE HIGH AFFINITY RECEPTOR SITE.
S. P. Lad, D. A. Peterson, R. A. Bradshaw, and K. E. Neet (2003)
J. Biol. Chem. 278, 24808-24817
   Abstract »    Full Text »    PDF »
Mitochondrial movement and positioning in axons: the role of growth factor signaling.
S. R. Chada and P. J. Hollenbeck (2003)
J. Exp. Biol. 206, 1985-1992
   Abstract »    Full Text »    PDF »
Four-dimensional organization of protein kinase signaling cascades: the roles of diffusion, endocytosis and molecular motors.
B. N Kholodenko (2003)
J. Exp. Biol. 206, 2073-2082
   Abstract »    Full Text »    PDF »
Ligand-Induced Internalization of the p75 Neurotrophin Receptor: A Slow Route to the Signaling Endosome.
F. C. Bronfman, M. Tcherpakov, T. M. Jovin, and M. Fainzilber (2003)
J. Neurosci. 23, 3209-3220
   Abstract »    Full Text »    PDF »
How does an axon grow?.
J. L. Goldberg (2003)
Genes & Dev. 17, 941-958
   Full Text »    PDF »
Trafficking of TrkA-Green Fluorescent Protein Chimerae during Nerve Growth Factor-induced Differentiation.
J. Jullien, V. Guili, E. A. Derrington, J.-L. Darlix, L. F. Reichardt, and B. B. Rudkin (2003)
J. Biol. Chem. 278, 8706-8716
   Abstract »    Full Text »    PDF »
Regulation of Receptor Tyrosine Kinase Signaling by Protein Tyrosine Phosphatase-1B.
F. G. Haj, B. Markova, L. D. Klaman, F. D. Bohmer, and B. G. Neel (2003)
J. Biol. Chem. 278, 739-744
   Abstract »    Full Text »    PDF »
Internalization of Inactive EGF Receptor into Endosomes and the Subsequent Activation of Endosome-Associated EGF Receptors.
Y. Wang, S. Pennock, X. Chen, and Z. Wang (2002)
Sci. STKE 2002, pl17
   Abstract »    Full Text »    PDF »
Cyclic AMP Induces Transactivation of the Receptors for Epidermal Growth Factor and Nerve Growth Factor, Thereby Modulating Activation of MAP Kinase, Akt, and Neurite Outgrowth in PC12 Cells.
A. Piiper, I. Dikic, M. P. Lutz, J. Leser, B. Kronenberger, R. Elez, H. Cramer, W. Muller-Esterl, and S. Zeuzem (2002)
J. Biol. Chem. 277, 43623-43630
   Abstract »    Full Text »    PDF »
Endosomal Signaling of Epidermal Growth Factor Receptor Stimulates Signal Transduction Pathways Leading to Cell Survival.
Y. Wang, S. Pennock, X. Chen, and Z. Wang (2002)
Mol. Cell. Biol. 22, 7279-7290
   Abstract »    Full Text »    PDF »
Local and Target-Derived Brain-Derived Neurotrophic Factor Exert Opposing Effects on the Dendritic Arborization of Retinal Ganglion Cells In Vivo.
B. Lom, J. Cogen, A. L. Sanchez, T. Vu, and S. Cohen-Cory (2002)
J. Neurosci. 22, 7639-7649
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)